Research Article | Volume: 8, Issue: 10, October, 2018

Dissolution profiles of partially purified bromelain from pineapple cores [Ananas comosus (L.) Merr] encapsulated in glutaraldehyde-crosslinked chitosan

Siswati Setiasih Hegi Adi Prabowo Emil Budianto Sumi Hudiyono   

Open Access   

Published:  Oct 31, 2018

DOI: 10.7324/JAPS.2018.81003
Abstract

To avoid degradation in the stomach, the proteolytic enzyme bromelain must be encapsulated in glutaraldehyde-crosslinked chitosan (CGF) hydrogels, which can maintain the activity of bromelain until it reaches the intestine. In this study, we isolated bromelain by using ammonium sulfate precipitation, dialysis, and anionic exchange chromatography with Diethylaminoethyl (DEAE)-cellulose resin. Bromelain fractions were collected from each purification step and specific activities were sequentially from increased in the crude enzyme, ammonium sulfate, dialysis, and DEAE chromatography fractions (fraction numbers 58–71), which have fractions of 23.90, 122.00, 125.48, and 195.20 U/mg, respectively. Bromelain fractions from the dialysis step were encapsulated in CGF matrixes by using a post-loading method. CGF hydrogels had a crosslinking degree of 84.37% and swelling ratio of 76.60%. The dissolution profiles of CGF-encapsulated bromelain were tested in artificial stomach fluid and intestinal environments, and bromelain encapsulation efficiency following the post-loading method was 96.29%. Interactions between the hydrogel and bromelain were limited to the hydrogen bonds, and the proteolytic activities of bromelain were maintained at 0.17 U/ml in the present artificial intestinal environment.


Keyword:     Bromelain purification post-loading encapsulation crosslinked chitosan dissolution.


Citation:

Setiasih S, Prabowo HA, Budianto E, Hudiyono S. Dissolution profiles of partially purified bromelain from pineapple cores [Ananas comosus (L.) Merr] encapsulated in glutaraldehydecrosslinked chitosan. J App Pharm Sci, 2018; 8(10): 017-024.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reference

Ataide JA, Cefali LC, Rebelo MdA, Spir LV, Tambourgi EB, Jozala AF, et al. Bromelain loading and release from hydrogel formulated using alginate and arabic gum. Planta Med, 2017; 83:870–6. https://doi.org/10.1055/s-0043-102948

Abdel-Mohzen AM, Aly AS, Hrdina R, Montaser AS, Hebeish A. Eco-synthesis of PVA/chitosan hydrogels for biomedical application. J Polym Environ, 2011; 19:1005–12. https://doi.org/10.1007/s10924-011-0334-0

Banerjee S, Siddiqui L, Bhattacharya S, Kaity S, Ghosh A, Chattopadhyay P, et al. Interpenetrating polymer network (IPN) hydrogel microspheres for oral controlled release application. Int J Biol Macromol, 2010; 50:198–206. https://doi.org/10.1016/j.ijbiomac.2011.10.020

Bernela M, Ahuja M, Thakur R. Enhancement of anti-inflamatory activity of bromelain by its encapsulation in katira gum nanoparticles. Carbohydr Polym, 2016; 143:18–24. https://doi.org/10.1016/j.carbpol.2016.01.055

Budianto E, Muthoharoh SP, Nizardo NM. Effect of crosslinking agents, pH and temperature on swelling behavior of cross-linked chitosan hydrogel. Asian J App Sci, 2015; 3:581–8.

Chobotova K, Vernallis AB, Majid FAA. Bromelain's activity and potential as an anti-cancer agent: current evidence and perspectives. Cancer Lett, 2010; 290:148–56. https://doi.org/10.1016/j.canlet.2009.08.001

Costa HB, Fernandesa PMB, Romão W, Ventura JA. A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies. Ind Crops Prod, 2014; 59:163–8. https://doi.org/10.1016/j.indcrop.2014.04.042

Croisfelt F, Martins BC, Rescolino R, Coelho DF, Zanchetta B, Mazzola PG, et al. Poly(N-Isopropylacrylamide)-co-acrylamide hydrogels for the controlled release of bromelain from agroindustrial residues of ananas comosus. Planta Med, 2015; 81:1719–26. https://doi.org/10.1055/s-0035-1557867

Devakate RV, Patil VV, Waje SS, Thorat BN. Purification and drying of bromelain. Sep Purif Technol, 2009; 64:259–64. https://doi.org/10.1016/j.seppur.2008.09.012

Gautam SS, Mishra SK, Dash V, Goyal AK, Rath G. Comparative study of extraction, purification and estimation of bromelain from stem and fruit of pineapple plant. Thai J Pharma Sci, 2010; 34:67–76.

Gonçalves VL, Laranjeira MCM, Fávere VT. Effect of crosslinking agents on chitosan microspheres in controlled release of diclofenac sodium. Polímeros Ciência Tecnologia, 2005; 15:6–12. https://doi.org/10.1590/S0104-14282005000100005

Green AA, Hugesh WL. Protein solubility on the basis of solubility in aqueous solutions of salt and organic solvents. Methods Enzymol, 1955; 1:67–90. https://doi.org/10.1016/0076-6879(55)01014-8

Ketnawa S, Chaiwut P, Rawdkuen S. Pineapple wastes: a potential source for bromelain extraction. Food Bioprod Proc, 2012; 90:385–91. https://doi.org/10.1016/j.fbp.2011.12.006

Morris G, Castile J, Smith A, Adams GG, Harding SE. Macromolecular conformation of chitosan in dilute solution: a new global hydrodynamic approach. Carbohydr Polym, 2009; 76:616–21. https://doi.org/10.1016/j.carbpol.2008.11.025

Musfiroh FF, Setiasih S, Handayani S, Hudiyono S, Ilyas NM. In vivo antiplatelet activity aggregation assay of bromelain fractionate by ethanol from extract pineapple core (Ananas comosus [L.] Merr). IOP Conf Ser Mat Sci Eng, 2018; 299:1–4. https://doi.org/10.1088/1757-899X/299/1/012017

Ou A, Bo I. Chitosan hydrogels and their glutaraldehyde-crosslinked counterparts as potential drug release and tissue engineering system-synthesis, characterization, swelling kinetics and mechanism. J Phys Chem Biophys, 2017; 3:1–7. https://doi.org/10.4172/2161-0398.1000256

Reddy GV, Reddy NS, Nagaraja K, Rao KSVK. Synthesis of pH responsive hydrogel matrices from guar gum and poly (acrylamide-co-acrylamidoglycolicacid) for anti-cancer drug delivery. J App Pharm Sci, 2018; 8:84–91.

Respati E. Outlook nenas. Pusat Data dan Sistem Informasi Pertanian Sekretariat Jenderal Kementerian Pertanian, Jakarta, Indonesia, 2016.

Selvakumaran S, Muhamad II, Razak SI. Evaluation of kappa carrageenan as potential carrier for floating drug delivery system: effect of pore forming agents. Carbohydr Polym, 2016; 135:207–14. https://doi.org/10.1016/j.carbpol.2015.08.051

Setiasih S, Darwis AAC, Dzikria V, Hudiyono S. Stability test of partially purified bromelain from pineapple (Ananas comosus (L.) Merr) core extract in artificial stomatch fluid. IOP Conf Ser Mater Sci Eng, 2018; 299:1–7.

Waterborg JH. The lowry method for protein quantitation. In: Walker JM (ed.). The protein protocols handbook second edition. Humana Press, New Jersey, pp 7–9, 2002. https://doi.org/10.1385/1-59259-169-8:7

Yadav HKS, Shivakumar HG. In vitro and in vivo evaluation of pH-sensitive hydrogel of carboxymethyl chitosan for intestinal delivery of theophylline. ISRN Pharm, 2012; 2012(article ID 763127):1–9.

Zhao HR, Wang K, Zhao Y, Pan LQ. Novel sustained-release implant of herb extract using chitosan. Biomaterials, 2002; 23:4459–62. https://doi.org/10.1016/S0142-9612(02)00162-X

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