Immunostimulatory Potential of Papain Encapsulated Solid Lipid Nanoparticles

Stimulation of immune function is the primary focus in the field of cancer metastasis treatment. Cancer cells are circulated through the lymphatic system to support metastasis to lymph nodes and adjacent organs. In order to treat metastatic cancer, the immune function should be reactivated to recognize the cancer cells against the natural killer activity and expose the cancer cells to the chemotherapy. Proteolytic enzymes have been explored in cancer therapy including the inhibition of metastasis and improve the body immune mechanism. To achieve this hypothesis, we proposed papain loaded solid nanoparticles to stimulate the immune mechanism by investigating the following; (i) measurement of human lymphoblast cells using TK6 Spleen lymphoblast cell line (ii) proliferation of T cells and secretion of cytokines (IL-2 and IL-4) using T-lymphocytes 8E5 cells (iii) determination of in-vitro cytokines production by activated macrophages. This research evidenced that papain loaded solid lipid nanoparticles possess significant immunostimulatory efficacy, thus it can be proposed as an adjuvant therapy for cancer metastasis.