Research Article | Volume: 8, Issue: 6, June, 2018

Antibiofilm activity of papain enzyme against pathogenic Klebsiella pneumoniae

Sara H. Mohamed Mahmoud S. M. Mohamed Mary S. Khalil Walaa S. Mohamed Mona I. Mabrouk   

Open Access   

Published:  Jun 29, 2018

DOI: 10.7324/JAPS.2018.8621

Infections caused by biofilm-embedded pathogens decrease the efficacy of traditional treatments and increase antibiotic tolerance. Most of the human bacterial infections are biofilm-associated. Therefore, this study aimed to investigate the antimicrobial as well as the antibiofilm activity of papain enzyme on drug-resistant biofilm producing Klebsiella pneumoniae. Effect of papain enzyme was tested on Klebsiella pneumonia planktonic cells as well as on the formation, eradication and cells viability of biofilm. Although no antimicrobial activity of papain enzyme was detected against planktonic cells, significant biofilm inhibition and eradication were recorded. Biofilm inhibition of different K. pneumonia strains ranged from (10.6-56.2%) at concentration 50 mg/mL and increased to (21.4-59.0%) at 100 mg/mL papain. Furthermore, noticeable biofilm eradication was recorded (7.7-54.9%) and (9.6-55.6%) at 50 mg/ mL and 100 mg/mL papain concentration respectively. Nevertheless, no significant activity was detected on biofilm cells viability. Scanning electron microscopy (SEM) confirmed the inhibitory and eradication activity of papain on K. pneumoniae biofilm. This study demonstrated for the first time that papain enzyme exerts an antibiofilm effect against drug-resistant K. pneumoniae, but no antibacterial activity was detected, suggesting its potential application as an antibiofilm agent in combination with traditional antibacterial agents.

Keyword:     Biofilm SEM Papain MDR Klebsiella pneumoniae.


Mohamed SH, Mohamed MSM, Khalil MS, Mohamed WS, Mabrouk MI. Antibiofilm activity of papain enzyme against pathogenic Klebsiella pneumoniae. J App Pharm Sci, 2018; 8(06): 163-168.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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