Research Article | Volume: 8, Issue: 6, June, 2018

Artocarpin isolated from Artocarpus heterophyllus heartwoods alters membrane permeability of Streptococcus mutans

Abdi Wira Septama Pharkphoom Panichayupakaranant   

Open Access   

Published:  Jun 29, 2018

DOI: 10.7324/JAPS.2018.8608

Artocarpin was successfully isolated from the heartwoods of A. heterophyllus. Objectives: The present study was conducted to determine the antimicrobial activity of artocarpin against cariogenic microbial including Streptococcus mutans, Escherichia coli, and Candida albicans. The activity of artocarpin on alteration of the cell membrane was also investigated. Methods: Standard microdilution method was applied to evaluate its antibacterial activity against tested microorganisms. Time-killing test was then performed to verify its antimicrobial effect. In order to know its effect on membrane permeability, bacteriolysis assay was selected, followed by determining loss of 260 nm absorbing material using UV-Vis spectrophotometer. Results: Artocarpin exhibited strong antibacterial against S. mutans with MIC and MBC values of 1.95 and 3.91 μg/mL, respectively. Time-killing curve showed that artocarpin at 2 × MIC (3.9 μg/ mL) and 4 × MIC (7.8 μg/mL) inhibited bacterial growth after 4 h incubation which was comparable to ampicillin as a positive control. Artocarpin 2 × MIC (3.9 μg/mL) and 4 × MIC (7.8 μg/mL) altered membrane permeability of S. mutans after 24 h exposure and led to release 260 nm absorbing material as an indicator of membrane cell damage. Conclusion: This finding indicated that artocarpin has high potency as antibacterial compound against S. mutans by reducing cell membrane permeability.

Keyword:     Artocarpin A. heterophyllus antimicrobial membrane permeability S. mutans.


Septama AW, Panichayupakaranant P. Artocarpin isolated from Artocarpus heterophyllus heartwoods alters membrane permeability of Streptococcus mutans. J App Pharm Sci, 2018; 8(06): 059-064.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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