Research Article | Volume: 8, Issue: 4, April, 2018

The Effect of Darapladib Therapy for the Expression of Lp-PLA2 in Dyslipidemia and Type 2 Diabetes Mellitus Atherosclerosis Model

Titin Andri Wihastuti Rosaria Dian Lestari Teuku Heriansyah   

Open Access   

Published:  Apr 29, 2018

DOI: 10.7324/JAPS.2018.8409
Abstract

Atherosclerosis is the main cause of mortality and morbidity globally. Lipoprotein-associated phospholipase A2 (Lp- PLA2) activity is suspected to have a significant role in atherosclerosis. 50 Sprague-Dawley Rats were divided into five groups: normal, dyslipidemia, Type 2 diabetes mellitus (T2DM), dyslipidemia with darapladib administration and T2DM with darapladib administration. These groups were divided into two serial times: 8 and 16 weeks. mRNA Lp-PLA2 was measured from blood and aortic tissue extraction. Aortic tissue Lp-PLA2 was measured by immunofluorescence. Lp-PLA2 expression in aortic tissue was consistently increased in dyslipidemia and T2DM. The expression of Lp-PLA2 enzymatic was significantly suppressed (p < 0.05) with the administration of darapladib especially in 8 weeks groups in both dyslipidemia and T2DM. The administration of darapladib in dyslipidemia and T2DM didn’t significantly suppress the expression of mRNA Lp-PLA2 in blood and aortic tissue. The failure of genetic expression suppression of Lp-PLA2 was found in both 8 weeks and 16 weeks groups. The expression of Lp-PLA2 protein also showed an inclined difference between dyslipidemia and T2DM. These results showed that administration of darapladib significantly decreased Lp-PLA2 protein but prone to increase the expression of mRNA Lp-PLA2 in blood and aortic tissue in dyslipidemia and T2DM model.


Keyword:     darapladib dyslipidemia atherosclerosis T2DM Lp-PLA2.


Citation:

Wihastuti TA, Lestari RD, Heriansyah T. The Effect of Darapladib Therapy for the Expression of Lp-PLA2 in Dyslipidemia and Type 2 Diabetes Mellitus Atherosclerosis Model. J App Pharm Sci, 2018; 8(04): 064-070.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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