Short Communication | Volume: 8, Issue: 3, March, 2018

Application of FTIR spectroscopy and multivariate calibration for analysis of curcuminoid in syrup formulation

Niken K. Prabaningdyah ,  ,  Sugeng Riyanto,  Abdul Rohman ,    
Niken K. Prabaningdyah1 , 2, Sugeng Riyanto1, Abdul Rohman1 , 3

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia.

2The National Agency of Drug and Food Control, District of Yogyakarta, Republic of Indonesia.

3Research Center of Halal Products, Gadjah Mada University, Yogyakarta, 55281, Indonesia.

Open Access   

Published:  Mar 30, 2018

DOI: 10.7324/JAPS.2018.8324
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Abstract

Curcuminoid, the main components in Curcuma species, is responsible for several biological activities including antioxidant and hepatoprotective effect, therefore fast and reliable analytical method is required for curcuminoid determination. The objective of this study was to develop Fourier transform infrared (FTIR) spectroscopy in combination with multivariate calibration of partial least square (PLS) and principle component regression (PCR) for quantitative analysis of curcumin (CUR) and desmethoxycurcumin (DMC) in syrup sample containing extract of Curcuma xanthorriza (temulawak). Syrup samples were directly scanned using FTIR spectrophotometer with attenuated total reflectance (ATR) sampling technique at wave numbers 4000–650 cm−1 and its spectra were correlated with contents of CUR and DMC determined using high performance liquid chromatography. PLS offered better prediction model for the relationship between actual values of CUR and DMC and FTIR predicted values using absorbances at wavenumbers of 3004–974 cm−1 than that using PCR. PLS calibration model yielded R2 of 0.9999 (calibration) and 0.9976 (for validation) for such correlation. The errors in calibration and validation expressed by root mean square error of calibration (RMSEC) and root mean square error of prediction (RMSEP) were low, i.e. 0.0014 (RMSEC) and 0.0017 (RMSEP), respectively. FTIR spectroscopy in combination with PLS provide fast, accurate and precise method for determination of CUR and DMC in syrup samples.


Keyword:     FTIR spectroscopy curcumin demethoxycurcumin partial least square syrup.

Citation:

Prabaningdyah NK, Riyanto S, Rohman A. Application of FTIR spectroscopy and multivariate calibration for analysis of curcuminoid in syrup formulation. J App Pharm Sci, 2018; 8(03): 172-179.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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