6-shogaol from ginger oleoresin loaded liposomes using DMPG-Na as a carrier enhances the in-vitro and in-vivo anticancer activity

Ginger oleoresin (GO) plays an important role on the attenuation of complications associated to the cancer therapy which is attributed to 6-shogaol (6-SGL). The major challenge in using 6-SGL for therapeutic applications is its poor aqueous solubility, low stability in GI and low bioavailability. Considering the potent anticancer nature of 6-SGL and its synergistic activity with other constituents in GO, there is a need to develop a suitable drug delivery system. Thus in the present study, 6-SGL rich GO (6-SRGO) was incorporated into liposomes by solvent injection technique using lipid (DMPG-Na) as carrier. The prepared 6-SRGO loaded liposomes (6-SRGO-LLPS) were characterized physically and chemically using FTIR, DSC, surface morphology, drug content (DC), encapsulation efficiency (EE), particle size (PS), zeta potential and further evaluated for stability study, in vitro release study, in vitro cytotoxicity study and in vivo anticancer activity in comparison with 6-SRGO. 32 factorial designs was used for optimization of formulation variables and the results of DC, EE and PS obtained were 89.92 ± 1.56, 79.36 ± 1.05 and 356.11 ± 4.07 respectively. Considering the above findings, 6-SRGO-LLPS exhibited significant in vitro (GI50- <10 μg/ml) and in vivo anticancer activity in comparison with 6-SRGO.