Research Article | Volume: 8, Issue: 1, January, 2018

Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells

Nor-Hidayah Mustafa Azizah Ugusman Juriyati Jalil Yusof Kamisah   

Open Access   

Published:  Jan 28, 2018

DOI: 10.7324/JAPS.2018.8123
Abstract

Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study aimed to evaluate the anti-inflammatory properties of P. speciosa empty pod extract in human umbilical vein endothelial cells (HUVECs). The empty pods were extracted by sequential fractionation with absolute ethanol and ethyl acetate. HUVECs were divided into four groups. HUVECs were exposed to tumor necrosis factor-α (TNF-α, 10 ng/mL) in the presence (25 μg/mL) or absence of Parkia speciosa extract. Quercetin (125 μM) served as the positive control, while another group that was not exposed to TNF-α acted as the negative control. The protein expressions of the inflammatory mediators, which were NF-κB p65, iNOS, COX-2 and VCAM-1, were significantly decreased in the P. speciosa and quercetin groups exposed to TNF-α. P. speciosa extract and quercetin also significantly reduced intracellular reactive oxygen species and nitric oxide levels as well as inducible nitric oxide synthase (iNOS) activity caused by TNF-α in HUVECs. In conclusion, P. speciosa empty pod extract exhibited potential anti-inflammatory properties against TNF-α-induced inflammation, possibly by modulating the NF-κB p65 pathway. The effects were comparable to that of quercetin.


Keyword:     NF-κB p65 vascular cell adhesion molecule reactive oxygen species inducible nitric oxide synthase cyclooxygenase-2.


Citation:

Mustafa NH, Ugusman A, Jalil J, Kamisah Y. Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells. J App Pharm Sci, 2018; 8 (01): 152-158.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
445 Views 61 Downloads 506 Total

Year

Month

Similar Articles


Warning: mysqli_fetch_array() expects parameter 1 to be mysqli_result, bool given in /home/japsonli/public_html/articlemodule/database.php on line 426

In vitro analysis of Aegle marmelos leaf extracts on skin pathogens

Garima Mathur, Nidhija Roy, Ashwani Mathur

Related Search

By author names