Research Article | Volume: 8, Issue: 1, January, 2018

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

Jéssica Brandão Reolon Maicon Brustolin Sandra Elisa Haas Eduardo André Bender Marcelo Donadel Malesuik Letícia Marques Colomé   

Open Access   

Published:  Jan 28, 2018

DOI: 10.7324/JAPS.2018.8120
Abstract

This study aimed to develop and validate a HPLC based analytical methodology for simultaneous determination of acyclovir and curcumin within microparticles. Chromatographic separation was achieved by employing a Fenomenex C18 column as stationary phase and a ternary mixture of acetonitrile, 0.1% phosphoric acid, and methanol (50:40:10) as the mobile phase. The validated method proved to be linear in the range of 0.5-30 μg.mL−1 and 0.5-20 μg.mL−1 for acyclovir and curcumin, respectively. Detection and quantification limits for acyclovir were, respectively, 83.62 ng.mL−1 and 109.52 ng.mL−1, while for curcumin the values were 91.61 ng.mL−1 and 128.71 ng.mL−1, what assures the methodology sensitivity. The method was also precise (1.2% RSD for acyclovir and 1.38% RSD for curcumin), besides showing recovery rates close to 100% for both of two drugs when accuracy was accessed. Minor alterations over chromatographic setup have confirmed methodology robustness. The present methodology proved to be capable of detecting and quantifying acyclovir and curcumin at polymeric microparticles in a single run, showing itself as an analytical alternative to be employed in the quality control for this dosage form.


Keyword:     HPLC method validation acyclovir curcumin microparticles.


Citation:

Reolon JB, Brustolin M, Haas SE, Bender EA, Malesuik MD, Colomé LM. Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles. J App Pharm Sci, 2018; 8 (01): 136-141.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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