Research Article | Volume: 7, Issue: 9, September, 2017

Expression of microRNAs-21 and-223 in hepatocellular carcinoma in hepatitis C virus infected Egyptian population

Eman A. Elghoroury,  Soheir A. Abdel Maksoud,  Dina Kandil,  Mona Raafat El Kafoury,  Eman Mahmoud Hassan,  Eman Awadallah,  Mohamed A. Hussein,  Hany Ahmed Elghobary   
Eman A. Elghoroury1, Soheir A. Abdel Maksoud1, Dina Kandil1, Mona Raafat El Kafoury1, Eman Mahmoud Hassan, Eman Awadallah1, Mohamed A. Hussein2, Hany Ahmed Elghobary3

1Clinical and Chemical Pathology Department, National Research Centre, El-Buhouth St., Dokki, Cairo, Egypt.

2Internal Medicine Department, Cairo University, Cairo, Egypt.

3Clinical and Chemical Pathology Department, Cairo university, Cairo, Egypt.

Open Access   

Published:  Sep 30, 2017

DOI: 10.7324/JAPS.2017.70907
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Abstract

Background: The prognosis of hepatocellular carcinoma (HCC) in Egypt is poor due to high prevalence of hepatitis C virus (HCV). Moreover, the performance of laboratory markers for diagnosis of HCC at early stages has not been optimal.



Objective: We aim to assess expression of miR -21 and miR-223 in HCC patients in the presence of HCV infection and correlate this expression with other clinical, laboratory features of HCC and other markers such as cyclase-associated protein2 (CAP2) and macrophage migration inhibitory factor (MIF).



Subjects and Methods: The study included 40 cirrhotic, 40 HCC patients and 40 healthy subjects. Assessment of miRNAs was done using RT-PCR (Applied Biosystems).



Results: Levels of miR-21 were higher in patient groups compared to control group and in HCC patients compared to other patients (p=0.001, 0.002 respectively). Serum levels of miR-223 showed no significant differences between cirrhotic patients and control group (p=0.2), while marked decrease in miR-223 expression levels was found in HCC patients compared to cirrhotic patients and control group (p=0.004,0.001 respectively).



Conclusion: Altered serum miR-21 and -223 may play role in pathogenesis of HCC in the presence of HCV infection and might serve as a potential biomarker for detection of early HCC.


Keyword:     CAP2HCVHCCMIFmicroRNAs.

Citation:

Elghoroury EA, Abdel Maksoud SA, Kandil D, El Kafoury MR, Hassan EM, Awadallah E, Hussein MA, Elghobary HA. Expression of microRNAs-21 and-223 in hepatocellular carcinoma on top of hepatitis C virus in a sample of Egyptian population. J App Pharm Sci, 2017; 7 (09): 052-057.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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