Research Article | Volume: 7, Issue: 9, September, 2017

Methionine and Choline Deficient Diet-induced Non-Alcoholic Steatohepatitis in Rats: Role of Melatonin

Mohamed M. AminRania F. AhmedDalia O. Saleh   

Open Access   

Published:  Sep 30, 2017

DOI: 10.7324/JAPS.2017.70902
Abstract

Objective: The present study aimed to evaluate the neuro-protective and hepato-protective activity of melatonin (MLN) on non-alcoholic steatohepatitis (NASH) in albino rats induced by methionine and choline deficient (MCD) diet.


Material and Methods: To achieve this aim, rats were fed MCD diet for 8 weeks to induce non-alcoholic steatohepatitis. Oral administration of MLN (10 mg/kg/day) was done for the last two consecutive weeks. Twenty-four hours after the last dose of the MLN behavioral test, namely activity cage test was performed and the activity counts were recorded. Serum AST, ALT, ALP, GGT, total/direct bilirubin and albumin were valued to assess liver function. Moreover, hepatic interleukin-6 (IL-6) as well as nuclear factor- kβ (NF-kβ) were determined. Besides, brain biomarkers viz. ammonia, nitric oxide and brain-derived neurotrophic factor (BDNF) were measured which are reliable indices to assess brain damage.


Results: MLN succeeded in suppression of NASH rats’ locomotor activity. Besides; it improved all biochemical parameters and histopathological as well as proliferating cell nuclear antigen (PCNA) immunohistochemical profiles of liver and brain.


Conclusions: These results afford new intuition into the possible molecular mechanisms by which MLN faces steatohepatitis disorder, via modulation of several factors participating in the progression of this disease.


Keyword:     MelatoninNon-alcoholic steatohepatitismethionine and choline deficient diet.


Citation:

Amin MM, Ahmed RF, Saleh DO. Methionine and Choline Deficient Diet-induced Non-Alcoholic Steatohepatitis in Rats: Role of Melatonin on Brain Derived Neurotrophic Factor. J App Pharm Sci, 2017; 7 (09): 012-019.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics

409 Absract views 27 PDF Downloads 436 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required