Research Article | Volume: 7, Issue: 7, July, 2017

Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

Tengku Haziyamin Abdul Hamid Azzmer Azzar Abdul Hamid   

Open Access   

Published:  Jul 30, 2017

DOI: 10.7324/JAPS.2017.70707
Abstract

E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures (TMP) through vacuum suction of 0.1 to 0.7 bar were applied to the plasmid sample, and the corresponding flow rates and fluxes for both filters were investigated. Even though, these two filters showed a slight different in the flux, a marked different in DNA transmission were observed. DNA transmission were generally higher with cellulose generated filters, whereby DNA transmission by Ultracel filter was at44.3, 63.9, 74.1, 55.6 and 54.5% at TMP of 0.1, 0.18, 0.39, 0.6 and 0.7 bar, respectively. Meanwhile, for PES filters the DNA transmission was at 22.3, 38.1, 39.1, 38.0 and 37.5% at TMP of 0.1, 0.3, 0.4, 0.6 and 0.7 bar, respectively. With the cellulose generated filter, DNA transmission reached an optimum (~70%) at about 0.6 bar after which the transmission depleted at higher TMP of 0.7 bar. Throughout all of the TMP, DNA transmissions observed were generally lower with PES filter. The properties of the filter material could have contributed to the differences in DNA permeation.


Keyword:     Plasmid purification membrane plasmid flux.


Citation:

Mostafa SW, El-Sersy NA, Abdelwahab AE, Ali MAR. Statistical optimization and valuable applications of bioactive compounds produced by marine Pseudoalteromonas piscicida. J App Pharm Sci, 2017; 7 (07): 046-050.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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