Research Article | Volume: 7, Issue: 6, June, 2017

Synthesis and evaluation of silver nanoparticles using Cymodocea rotundata against clinical pathogens and human osteosarcoma cell line

Jayanthi Abraham Shantanu Saraf Vakas Mustafa Yugal Chaudhary Silambarasan Sivanangam   

Open Access   

Published:  Jun 30, 2017

DOI: 10.7324/JAPS.2017.70608
Abstract

Objective: To evaluate the silver nanoparticles (AgNPs) synthesis by aqueous extract of Cymodocea rotundata against clinical pathogens and human osteosarcoma cell line. Methods: In the present study, AgNPs were synthesized by Cymodocea rotundata by reducing 1 mM silver nitrate (AgNO3) solution. The green synthesized AgNPs were characterized by UV-vis spectroscopy, Atomic force microscopy (AFM), Scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Results: FTIR spectroscopy confirmed the presence of protein as the stabilizing agent surrounding the AgNPs. Morphologically, the nanoparticles were found to be spherical with an average particle size distribution of 24.2 nm. The green synthesized AgNPs showed powerful antioxidant properties in in-vitro antioxidant assays and effective in inhibiting human pathogens. The AgNPs also showed potent cytotoxic effect against MG63 cell lines with an IC50 value of 25.31 μg ml-1 which was confirmed by MTT assay. Conclusions: The results support The advantage of using bio-green method for synthesizing AgNPs with antioxidant, antimicrobial and cytotoxic activities are simple and cost effective.


Keyword:     Silver nanoparticles Cymodocea rotundata FTIR SEM MG63 cell line.


Citation:

Abraham J, Saraf S, Mustafa V, Chaudhary Y, Sivanangam S. Synthesis and evaluation of silver nanoparticles using Cymodocea rotundata against clinical pathogens and human osteosarcoma cell line. J App Pharm Sci, 2017; 7 (06): 055-061.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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