Research Article | Volume: 7, Issue: 6, June, 2017

A systematic multicellular spheroids screening approach lead to the identification of antineoplastic activity in three different plant extracts from the Egyptian flora

Walid Fayad,  Salwa M. El-Hallouty,  May A. El-Manawaty,  Marwa M. Mounier,  Ahmed A. F. Soliman,  Abeer A. Yousry,  Ayman A. Farghaly,  Maha A. Fahmy,  Zeinab M. Hasasn,  Stig Linder ,  Khaled Mahmoud   
Walid Fayad1, Salwa M. El-Hallouty1, May A. El-Manawaty1, Marwa M. Mounier1, Ahmed A. F. Soliman1, Abeer A. Yousry2, Ayman A. Farghaly3, Maha A. Fahmy3, Zeinab M. Hasasn4, Stig Linder5 & 6, Khaled Mahmoud1

1Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, Pharmaceutical and Drug Industries Division, National Research Centre, Dokki, Giza, 12622, Egypt.

2Medicinal and Aromatic Plants Department, Pharmaceutical and Drug Industries Division, National Research Centre, Dokki, Giza, 12622, Egypt.

3Genetics and Cytology Department, National Research Centre, Dokki, Giza, 12622, Egypt.

4Chemistry of Natural Compounds Department, National Research Centre, Dokki, Giza, Egypt.

5Department of Medical and Health Sciences, Linköping University, Linköping, Sweden.

6Cancer Center Karolinska, Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden.

Open Access   

Published:  Jun 30, 2017

DOI: 10.7324/JAPS.2017.70603
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Abstract

Developing natural products as potential antineoplastic drugs is a meticulous process involving both compound isolation and biological testing. Many studies are based on primary screening using tumor cell viability as the readout followed by compound isolation. We here present an approach which utilizes both 2-D and 3-D cultured of tumor cells for screening and immortalized human non-transformed cells for counter screening. This procedure increases the precision of identifying tumor-specific cytotoxic compounds with interesting pharmacological properties. Using this straight-forward approach, we screened 500 plant extracts from the Egyptian flora for anticancer activity. The primary screen on 2-D cultured cells yielded 41 hits, 12 of which showed significant cytotoxicity on 3-D cultured cells. Of these, 4 extracts showed limited cytoxicity to normal cells. We conclude that only ~10% of the cytotoxic extracts showed desired properties with regard to tumor parenchyme penetration and tumor-specific activity. Extracts from Euphorbia dendroides L. herb, Ononis vaginalis Vahl. herb and Quercus robur L. branches were found to induce tumor apoptosis and were considered the most promising. These three extracts showed significant inhibition in the Ehrlich ascites carcinoma in vivo model and did not show severe toxicity on healthy animals.


Keyword:     Anticancer Plant extracts Multicellular spheroids Screening Pro-apoptotic Therapeutic window.

Citation:

Fayad W, El-Hallouty SM, El-Manawaty MA, Mounier MM, Soliman AFM, Yousry AA, Farghaly AA, Fahmy MA, Hasasn ZM, Linder S, Mahmoud K. A systematic multicellular spheroids screening approach lead to the identification of antineoplastic activity in three different plant extracts from the Egyptian flora. J App Pharm Sci, 2017; 7 (06): 013-022.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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