Research Article | Volume: 7, Issue: 5, May, 2017

Cistanche tubulosa induces reactive oxygen species-mediated apoptosis of primary and metastatic human colon cancer cells

Afnan Saleh Al-Menhali Safya Ali Jameela Aishah A. Latiff Mohamed A. Elrayess Mohammed Alsayrafi Morana Jaganjac   

Open Access   

Published:  May 30, 2017

DOI: 10.7324/JAPS.2017.70507
Abstract

Colon cancer is the third most common cancer worldwide. Conventional therapies have shown moderate efficacy with severe adverse effects, therefore there is an urgent need for safer alternatives. In this study, Cistanche tubulosa, local name Thanoon, was considered as a potential phytotherapeutic strategy because of its known high therapeutic potential in traditional medicine and wide abundance in the Middle East region. Bioactive compounds were extracted from powdered Cistanche tubulosa and tested for their anticancer properties against four colon cancer cell lines including two derived from primary tumor (CaCo2 and HCT116) and two derived from metastatic site (LoVo and SW620). Effect of Cistanche tubulosa on induction of apoptosis and cellular redox homeostasis were also investigated. Cistanche tubulosa exhibited a concentration and time-dependent inhibition of proliferation of all tested cancer cell lines by more than 60% upon 72 hours treatment with 1 mg/mL of crude extract. Inhibition of proliferation was marked by induction of apoptosis, intracellular reactive oxygen species production and mitochondrial superoxides. This data suggest that Cistanche tubulosa is a promising candidate for additive anti-colon cancer therapy. This is the first study showing anticancer bioactivity of Cistanche tubulosa against colon cancer cells.


Keyword:     Cistanche tubulosa colon cancer redox homeostasis anticancer bioactivity.


Citation:

Al-Menhali AS, Jameela SA, Latiff AA, Elrayess MA, Alsayrafi M, Jaganjac M. Cistanche tubulosa induces reactive oxygen species-mediated apoptosis of primary and metastatic human colon cancer cells. J App Pharm Sci, 2017; 7 (05): 039-045.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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