Research Article | Volume: 7, Issue : 4, April, 2017

Phytochemical screening and evaluation of antioxidant and antibacterial activities of seeds and pods extracts of Calycotome villosa subsp. Intermedia

Aouicha Elkhamlichia,  Hanane El Hajajia,  Hassan Farajb,  Anouar Alamib,  Brahim El Balia,  Mohammed Lachkara   
Aouicha Elkhamlichia, Hanane El Hajajia, Hassan Farajb, Anouar Alamib, Brahim El Balia, Mohammed Lachkara

aEngineering Laboratory of Organometallic and Molecular Materials (CNRST–URAC19), Faculty of Sciences, Sidi Mohammed Ben Abdellah University, Po. Box 1796 (Atlas), 30000 Fez, Morocco.

bLaboratory of Organic Chemistry, Faculty of Sciences, Sidi Mohammed Ben Abdellah University, Po. Box 1796 (Atlas), 30000 Fez, Morocco.

Open Access   

Published:  Apr 30, 2017

DOI: 10.7324/JAPS.2017.70428
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Abstract

In the present study, antioxidant potential of the methanol and the ethyl acetate extracts of the seeds and pods of Calycotome villosa subsp. intermedia were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. In DPPH scavenging assay, the IC50 value of the methanolic extract of seeds was found to be 0.20 mg/mL, the standard reference value for butylatedhydroxytoluene (BHT) is 0.19 mg/mL. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, Calycotome villosa extract showed strong reducing power. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 173.37 ± 0.02 to 332.23 ± 0.01 mg GA (Gallic Acid)/g of dry extract. The concentrations of flavonoids in the extracts varied from 5.02 ± 0.03 to 66.45 ± 0.01 Qu (Quercetin)/g of dry extract. Ethyl acetate extract of seeds of Calycotome villosa showed the highest phenolic, flavonoids concentration and the strongest antioxidant activity. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant activity. Furthermore, all extracts showed good antibacterial activity against Escherichia coli. Calycotome villosa subsp. intermedia can be regarded as promising candidate for natural plant sources of antioxidants with high value.


Keyword:     Calycotome villosa subsp. intermedia antioxidant activity total flavonoids contents total phenolic contents antibacterial activity.

Citation:

Elkhamlichi A, El-Hajaji H, Faraj H, Alami A, El-Bali B, Lachkar M. Phytochemical screening and evaluation of antioxidant and antibacterial activities of seeds and pods extracts of Calycotome villosa subsp. Intermedia. J App Pharm Sci, 2017; 7 (04): 192-198.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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