Research Article | Volume: 7, Issue : 4, April, 2017

The Activity of Cellulase Enzymes of Ganoderma applanatum and Ganoderma tropicum

Nyi Mekar Saptarini Ginayanti Hadisoebroto   

Open Access   

Published:  Apr 30, 2017

DOI: 10.7324/JAPS.2017.70419
Abstract

Ganoderma can live as saprobes on rotting stumps and roots by decomposing lignin as well as cellulose and related polysaccharides. It is because of mushrooms cellulolytic activities.

Objective: The aim of this study was to compare the cellulolytic activity of the crude extract of Ganoderma applanatum and Ganoderma tropicum from Garut (Indonesia).

Method: Colorimetric method using dinitrosalicylic acid (DNS).

Results: The cellulolytic activity of G. applanatum and G. tropicum in the pH range of 3.5-10.0 was 0.051-0.109 and 0.063-0.131 UI/mL, respectively. The cellulolytic activity of G. applanatum and G. tropicum in the temperature range of 35-65 °C was 0.100-0.184 and 0.077-0.110 UI/mL, respectively.

Conclusion: The activity of cellulase enzymes were affected by temperature and pH. The cellulolytic activity of G. applanatum was the same as G. tropicum.


Keyword:     Cellulolytic colorimetric DNS various pH various temperature.


Citation:

Saptarini NM, Hadisoebroto G. The Activity of Cellulase Enzymes of Ganoderma applanatum and Ganoderma tropicum. J App Pharm Sci, 2017; 7 (04): 134-137.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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