Research Article | Volume: 7, Issue: 3, March, 2017

Acute and sub-acute toxicity of Harungana madagascariensis LAM (Hypericaceae) stem bark methanol extract

Rebecca Madeleine Ebelle Etame,  Raymond Simplice Mouokeu,  Rosalie Anne Ngono Ngane,  Jean Paul Assam Assam,  Azaber Mamoune Masoohe,  Raphael Tientcheu,  Mylene Laure Hopogap,  François Xavier Etoa   
Rebecca Madeleine Ebelle Etame1, Raymond Simplice Mouokeu2, Rosalie Anne Ngono Ngane3, Jean Paul Assam Assam1, Azaber Mamoune Masoohe1, Raphael Tientcheu1, Mylene Laure Hopogap3, François Xavier Etoa3

1Institute of Fisheries and Aquatic Sciences, University of Douala, Po Box 7236, Douala, Cameroon.

2Laboratory of Biochemistry, Department of Biochemistry, Faculty of Science, University of Douala, Po Box 24157, Douala, Cameroon.

3Department of Microbiology, University of Yaoundé I, Faculty of Sciences, Po box 812, Yaoundé, Cameroon.

Open Access   

Published:  Mar 30, 2017

DOI: 10.7324/JAPS.2017.70326
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Abstract

The adverse effect of H. madagascariensis bark methanol extract was investigated. The extract was prepared by maceration of dry powder. For acute toxicity study, a single oral dose of 5000 mg/kg body weight was given to rats and observed for 14 days. In sub-acute toxicity, rats were treated with 200, 400 and 800 mg/kg for 28 days. Body weights and behavior were noted throughout the experiment. Upon treatment, blood and urine were collected for hematological and biochemical analysis. Liver, lungs, heart, kidneys, testes and ovaries were analyzed for relative weights and histopathology. The LD50 value was greater than 5000 mg/Kg. In sub-acute treatment, H. madagascariensis extract caused a decrease in neutrophils, monocytes, red blood cells and platelets number. Biochemical parameters showed an increase in total cholesterol, LDL, urinary proteins, glucose and creatinin elimination speed while a reduction in triglycerides, HDL, serum glucose, bilirubin and transaminases activity were noted. An increase in liver and heart relative weights was observed. Histopathological analysis revealed urinary tract constriction, liver vascular constriction and heart, lungs, ovaries and testes necrosis. The study provides valuable data on the acute and sub-acute oral toxicity of H. madagascariensis that could be very useful for future assay.


Keyword:     Harungana madagascariensis acute toxicity sub-acute toxicity biochemical parameters hematological parameters.

Citation:

Etame RME, Mouokeu RS, Ngane RAN, Assam JP, Masoohe AM, Tchientcheu R, Hopogap MLM, Etoa FX. Acute and sub-acute toxicity of Harungana madagascariensis LAM (Hypericaceae) stem bark methanol extract. J App Pharm Sci, 2017; 7 (03): 160- 167.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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