Research Article | Volume: 7, Issue: 3, March, 2017

Application of modern RP-HPLC technique for the quantitation of betulinic acid from traditional drug Symplocos racemosa Roxb.

Sunita Shailajan Sasikumar Menon Dipti Singh Gauri Swar Suhina Bhosale   

Open Access   

Published:  Mar 30, 2017

DOI: 10.7324/JAPS.2017.70321

Objective: Symplocos racemosa Roxb. is traditionally used in various ailments like leprosy, diarrhoea, dysentery, menorrhagia and uterine disorders etc. The plant exhibits these therapeutic effects due to the presence of different biologically active phytoconstituents like ellagic acid, symplocoside, betulin, oleanolic acid, β-sitosterol, betulinic acid, etc. The bark has been reported to possess antiandrogenic, anticancer, antioxidant, antiulcer, hepatoprotective, angiogenic activity, in gynecological disorders etc. Therefore, the bark of S. racemosa has been an important ingredient of many traditional and Ayurvedic formulations like Rodhrasava, Pushyanug churna, Dashmularista, etc. The present study aims to develop and validate an efficient RP-HPLC method for the quantification of betulinic acid from the bark of Symplocos racemosa and an Ayurvedic formulation. Material and Methods: Separation was achieved on C18-column using acetonitrile: distilled water (85:15, v/v) as the mobile phase at a flow rate of 1 mL/min. The detection was carried out at 210 nm. Result and Conclusion: The linearity range was 5-150 µg/mL. The content of betulinic acid in Pushyanug churna was found to be 2.55±0.039 mg/g. The validated method was found to be simple, sensitive, accurate, rugged and reproducible. The developed method can be recommended for marker-based standardization of traditional formulations containing bark of S. racemosa.

Keyword:     Symplocos racemosa Lodhra Betulinic acid RP-HPLC Validation.


Shailajan S, Menon S, Singh D, Swar G, Bhosale S. Application of modern RP-HPLC technique for the quantitation of betulinic acid from traditional drug Symplocos racemosa Roxb. J App Pharm Sci, 2017; 7 (03): 129-134.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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