Research Article | Volume: 7, Issue: 3, March, 2017

Inhibition of Mitochondrial Respiratory Chain Activity by O, O-Dimethyl- and 4-O- Methyl- Alpinumisoflavones

Augustine Ocloo Robert Kingsford-Adaboh Andrew J. Murray   

Open Access   

Published:  Mar 30, 2017

DOI: 10.7324/JAPS.2017.70315
Abstract

Although the molluscicidal activity of crude dichloromethane extract of Millettia thonningii, known to be rich in alpinumisoflavones was attributed to its inhibition of isolated rat liver mitochondrial complex I activity, the effects of the constituent alpinuisoflavones on mitochondrial function have not been reported. The present study, therefore investigated the effect of O, O-dimethyl-alpinumisoflavone, a constituent of the crude dichloromethane extract of M. thonningii seeds and 4'-O-methyl-alpinumsioflavone, a naturally occurring alpinumisoflavone in Lonchocarpus glabrescens in isolated rat liver mitochondria and permeabilised mouse heart fibers using substrate-inhibitor titrations. The O, O-dimethyl-alpinumisoflavone inhibited State 3 respirations supported by glutamate/malate (complex I), succinate (complex II) and Ascorbate/TMPD (complex IV) in the permeabilised cardiac tissues but inhibited only the State 3 respiration supported by glutamate (complex I) in the isolated rat liver mitochondria. The 4'-O-methyl-alpinumsioflavone on the other hand inhibited State 3 respirations supported by all the substrates in both the isolated rat liver mitochondria and the permeabilised cardiac tissues. Thus, whilst O, O-dimethyl-alpinumisoflavone, a naturally occurring phyto-compound in the extract of M. thonningii seeds inhibits mitochondrial respiratory chain activity essentially at complex I, its derivative 4'-O-methyl-alpinumisoflavone, a naturally occurring phytocompound in Lonchocarpus glabrescens inhibits mitochondrial respiratory complexes I, II and IV.


Keyword:     Mitochondrial respiratory complexes inhibition alpinumisoflavones.


Citation:

Ocloo A, Kingsford-Adaboh R, Murray AJ. Inhibition of Mitochondrial Respiratory Chain Activity By O, O-Dimethyl- and 4-O- Methyl- Alpinumisoflavones. J App Pharm Sci, 2017; 7 (03): 095-100.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics

420 Absract views 9 PDF Downloads 429 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required