Research Article | Volume: 7, Issue: 3, March, 2017

Effect of amygdaloid complex inhibition on nicotine-induced conditioned place preference in rats

Sara Sadeghi-Gharajehdaghi,  Hedayat Sahraei,  Zahra Bahari,  Gholam Hossein Meftahi,  Gila Pirzad Jahromi,  Hengameh Ali-Beik   
Sara Sadeghi-Gharajehdaghi1, Hedayat Sahraei2, Zahra Bahari3, Gholam Hossein Meftahi2, Gila Pirzad Jahromi2, Hengameh Ali-Beik1

1Department of Biology, Faculty of Science, Islamic Azad University, North Tehran Branch, Tehran, Iran.

2Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

3Department of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

Open Access   

Published:  Mar 30, 2017

DOI: 10.7324/JAPS.2017.70307
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Abstract

Nicotine dependence is the major cause of cigarette smoking. The dependence occurred in the brain mesolimbic system and the amygdala has an important role in its initiation. Nicotine can activate dopaminergic neurons within the amygdaloid complex However, there is no evidence about complete inhibition of amygdala on nicotine dependence, thus in the present study we used conditioned-place preference (CPP) method to study the effect of transient inhibition of left and/or right side of the amygdala by lidocaine on nicotine reward properties in male Wistar rats. One week after two side cannulation and recovery the rats were conditioned to nicotine. Lidocaine hydrochloride (2%) was injected in amygdaloid complex, five min before each nicotine intraperitoneal (IP) injection for transient inhibition. On the test day, the animals were placed in the apparatus and their behaviors were recorded by a camera for 600 seconds. Then the records were analysis off line for sniffing, rearing, locomotion and compartment crossing. Three-way analysis of variance (ANOVA) was performed for statistical analysis. Our results showed that intra-peritoneal injection of nicotine (1 and 1.5 mg/kg) induced place preference. Transient inhibition of left or /and right side of the amygdaloid complex reduces nicotine place conditioning. In addition, rearing and compartment crossing also were reduced. However, when left side of the nucleus was inhibited, sniffing was increased, but when the right side was inhibited the sniffing was decreased. In conclusion, these results confirmed the involvement of both left and right sides of the amygdaloid complex in nicotine place conditioning.


Keyword:     Amygdaloid Complex Compartment crossing Lidocaine Nicotine Rearing Sniffing Rat.

Citation:

Sadeghi-Gharajehdaghi S, Sahraei H, Bahari Z, Meftahi GH, Jahromi GP, Ali-Beik H. Effect of amygdaloid complex inhibition on nicotine-induced conditioned p

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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