Research Article | Volume: 7, Issue: 2, February, 2017

In vitro α-amylase and α-glucosidase inhibitory activity of Ononis angustissima extracts

Hind Laoufi Nabila Benariba Sara Adjdir Rabah Djaziri   

Open Access   

Published:  Feb 27, 2017

DOI: 10.7324/JAPS.2017.70227
Abstract

The purpose of present study was to investigate the effects of extracts of O. angustissima a traditional medicinal plant, on α-amylase and α-glucosidase activities in vitro. The air-dried aerial parts of O. angustissima flowers, leaves and stems were extracted with distilled water (AE) and aqueous methanol (MeOH-H2O); ethyl acetate (EtAOc) and n-butanol extracts (n-BuOH) were obtained from MeOH-H2O crude extracts per liquid/liquid extraction. The results of the both enzyme inhibition activity was found in a dose-dependent manner, The strongest activity in case of α- glucosidase was shown by MeOH-H2O and n-BuOH extracts of O. angustissima with IC50 value 0.94 and 0.99 mg/mL, respectively, whereas AE and EtOAc extract showed 1.10 and 1.17 mg/mL as IC50 value respectively compared with the standard acarbose having IC50 value 0.046 mg/mL. Whereas the higher activity in case of α-amylase inhibition, was found in MeOH-H2O extract (IC50 = 2.01 mg/mL) followed by AE (2.52 mg/mL) and n-BuOH extract (2.88 mg/mL) respectively, compared with acarbose having IC50 value of 0.044 mg/mL. All extract from this plant possess moderate α-amylase inhibition with potent α-glucosidase inhibitory activity which may offer better therapeutic strategy to minimized postprandial hyperglycemia and its complications.


Keyword:     α- glucosidase inhibition α-amylase inhibition Fabaceae Ononis angustissima polyphenols flavonoids.


Citation:

Laoufi H, Benariba N, Adjdir S, Djaziri R. In vitro α-amylase and α-glucosidase inhibitory activity of Ononis angustissima extracts. J App Pharm Sci, 2017; 7 (02): 191-198.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
465 Views 41 Downloads 506 Total

Year

Month

Related Search

By author names