Research Article | Volume: 7, Issue: 2, February, 2017

Application of Hantzsch Reaction as a New Method for Spectrofluorimetric Determination of Some Cephalosporins

Mohamed A. El-Hamda Ramadan Alia Ahmed M. Haredyb Hytham M. Ahmedc Sayed M. Derayeab Osama H. Abdelmageedb d   

Open Access   

Published:  Feb 27, 2017

DOI: 10.7324/JAPS.2017.70220

A selective spectrofluorimetric method was described for determination of some cephalosporins namely, cefadroxil, cefaclor, cefixime, cefpodxime, and cefepime. These drugs were utilized as a source of amine group to react with acetylacetone and formaldehyde reagents via a Hantzsch reaction. The fluorescence intensity of the reaction products were measured at an emission wavelength of482 nm after excitation at a wavelength of 415 nm. The linear calibration graphs were obtained in the concentration ranges of 1-100 µg/mL for either cefadroxil or cefaclor and 1-50 µg/mL for the other three drugs. The limits of detection and quantitation were in the concentration range of 0.31-0.82 and 0.72-2.5 µg/mL, respectively. The percent of relative standard deviation (at 50 µg/mL concentration level, n = 5) were in the range of 0.41-1.70% and 1.51-1.95% for intra-day and inter-day precision, respectively. The method was successfully applied for the assay of some dosage forms of the cited drugs and the results obtained were comparable with those obtained by a reported method.

Keyword:     Spectrofluorimetric method Cephalosporins Acetylacetone Formaldehyde Hantzsch reaction Dosage forms.


Mohamed A. El Hamd, Ramadan Ali, Ahmed M. Haredy, Hytham M. Ahmed, Sayed M. Derayea and Osama H. Abdelmageed., Application of Hantzsch Reaction as a New Method for Spectrofluorimetric Determination of Some Cephalosporins. J App Pharm Sci, 2017; 7 (02): 147-155.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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