Research Article | Volume: 7, Issue: 2, February, 2017

Validated Ultraviolet-Spectrometric Method for Determination of Sofosbuvir in Tablets Formulation

Mohamed A. El Hamd Ramadan Ali Adel A. Marzouk Osama H. Abdelmageed   

Open Access   

Published:  Feb 27, 2017

DOI: 10.7324/JAPS.2017.70214
Abstract

A simple, direct and rapid ultraviolet spectrometric method was developed and validated for determination of sofosbuvir (SOF) in its tablets formulation. SOF was dissolved in methanol and measured in ultraviolet region at λmax of 260 nm. The linearity of the calibration curve was over the concentration range of 5 - 100 μg/mL with a correlation coefficient of 0.999. The sensitivity was checked as the limit of detection and limit of quantification which were found to be 1.6 and 4.8 µg/mL respectively. The percentage recoveries of SOF, after extraction from its tablets, were ranged from 98.23 ± 1.04% – 99.38 ± 0.40% with a relative standard division less than 2.0% indicating acceptable accuracy and precision of the developed method. Further studies of the accuracy and precision of the proposed method were performed using standard addition method; the mean quantitative recovery of such studies were found to be in the range 97.99 ± 0.32 to 99.69 ± 0.15 with RSD ≤ 2.0%. These levels of accuracy and precision obtained indicate suitability of the developed method for the quality control analysis of the SOF in its tablets formulation.


Keyword:     Sofosbuvir tablets formulation Quality control analysis UV Spectrophotometry.


Citation:

El-Hamd MA, Ali R, Marzouk AA, Abdelmageed OH. Validated Ultraviolet-Spectrometric Method for Determination of Sofosbuvir in Tablets Formulation. J App Pharm Sci, 2017; 7 (02): 114-119.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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