Research Article | Volume: 7, Issue: 1, January, 2017

Preparative HPLC fractionation of Cinnamomum cassia Water Extract and their in-vitro Antimalarial Activities

Mutaz Akkawi Saleh Abu-Lafi Hanadi Attieh Qassem Abu-Remeleh Sadam Makhamra Mutaz Qutob   

Open Access   

Published:  Jan 31, 2017

DOI: 10.7324/JAPS.2017.70117
Abstract

Due to the development of antimalarial resistance strains of Plasmodium falciparum, the search for new antimalarial drugs is immediately needed. Such drugs might work by obstructing the heme detoxification pathway in the malaria parasite, a vital requirement for parasite existence in host erythrocytes. The present study is aimed to investigate the antimalarial activity of four preparative HPLC fractions from Cinnamomum cassia water extract using semi-quantitative in vitro micro-assay that is based on the inhibition of ferriprotoporphyrin IX (FP) biomineralization. A reversed phase C18 inch preparative column and water-acetonitrile binary solvent mixture mobile phase were used to collect the fractions at 275 nm. UHPLC-MS revealed that the major compounds in water crude Cinnamomum cassia extract are coumarin and cinnamic acid. Containing coumarin as a major compound, fraction 2 showed superior efficacy compared to chloroquine and 2-mercaptopyrimidine positive controls. Fraction 3 which contains cinnamic acid showed moderate activity, while fraction 4 was the least potent.


Keyword:     Preparative HPLCCinnamomum cassiaMalariaβ-HematinHemozoinChloroquine.


Citation:

Akkawi M, AbuLafi S, Attieh H, Abu-Remeleh Q, Makhamra S, Qutob M. Preparative HPLC fractionation of Cinnamomum cassia Water Extract and their in-vitro Antimalarial Activities. J App Pharm Sci, 2017; 7 (01): 129-134.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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