Research Article | Volume: 7, Issue: 1, January, 2017

Purification and Characterization of Extracellular Lipase from Staphylococcus epidermidis (MTCC 10656)

Sirisha Edupuganti Lakshmi Parcha Lakshmi Narasu Mangamoori   

Open Access   

Published:  Jan 31, 2017

DOI: 10.7324/JAPS.2017.70108
Abstract

Lipases are hydrolytic enzymes with wide range of industrial applications. In the present study Staphylococcus epidermidis strain L2 with ability to produce extracellular lipase enzyme has been isolated from cloth samples collected from the diary industry. The extracellular lipase enzyme has been purified to homogeneity. The purified enzyme has shown a specific activity of 123.95U/mg. The molecular weight of the purified lipase enzyme was found to be 28KDa. The pH and temperature optima of the enzyme were recorded as 7.5 and 40°C respectively. The lipase enzyme has shown stability and activity in presence of varying concentrations of emulsifier and different substrates.


Keyword:     Extracellular lipase Staphylococcus purification.


Citation:

Edupuganti S, Parcha L, Mangamoori LN. Purification and Characterization of Extracellular Lipase from Staphylococcus epidermidis (MTCC 10656). J App Pharm Sci, 2017; 7 (01): 057- 063.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics

391 Absract views 19 PDF Downloads 410 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required