Published:  Jan 31, 2017DOI: 10.7324/JAPS.2017.70106
L-asparaginase is an anti-tumor enzyme and widely accepted as chemotherapeutic agent which has activity against acute lymphoblastic leukemia. The current study targets the production of L-asparaginase by Pseudonocardia endophytica VUK-10 by a statistically designed model. Experiments were performed according to central composite design of RSM with five independent variables such as time, pH, temperature, concentrations of maltose and L-asparagine concentration for optimization. All the five conditions had significant interaction with other variables for the maximum response (L-asparaginase production). Maximum L-asparaginase production was recorded as 7.42 IU/ml slightly higher than the model predicted value of 6.8 IU/ml, from statistical optimization studies. An unstructured kinetic model was proposed to depict the profiles of biomass, substrate utilization and L-asparaginase production in optimized medium under shake flask level. The logistic and Leudeking-Piret expressions were modified to predict the kinetic model parameters (µmax, X0, Xmax, α, β, γ and η) and we found that L-asparaginase production was growth-associated. High significant correlation (R2) values of 0.86, 0.96 and 0.94 were observed with the experimental and predicted results for Pseudonocardia endophytica VUK-10 growth, L-asparaginase activity and Maltose utilization, respectively. The results obtained from medium optimization using RSM and unstructured mathematical models describe the L-asparaginase fermentation kinetics more effectively.
Managamuri U, Vijayalakshmi M, Gandur VSRK, Satish Babu R, Poda S. Optimization of culture conditions by Response Surface Methodology and Unstructured kinetic modeling for LAsparaginase production by Pseudonocardia endophytica VUK-10. J App Pharm Sci, 2017; 7 (01): 042-050.
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