Research Article | Volume: 7, Issue: 1, January, 2017

Anti-inflammatory action of seed extract and polymeric nanoparticles of Syzygium cumini in diabetic rats infected with Candida albicans

Paula E. R. Bitencourt Lariane O. Cargnelutti Carolina S. Stein Raquel Lautenchleger Luana M. Ferreira Manuela Sangoi Aline Boligon Marta M. M. F. Duarte Rafael N. Moresco Letícia Cruz Régis A. Zanette Sydney H. Alves Maria Beatriz Moretto   

Open Access   

Published:  Jan 31, 2017

DOI: 10.7324/JAPS.2017.70102
Abstract

The role of Syzygium cumini against chronic complications of Diabetes mellitus (DM), such as fungal infection and inflammation, has been poorly explored. Here, we evaluated the treatment with S. cumini aqueous seed extract (ASc, 100mg/Kg) and polymeric nanoparticles containing ASc (NPASc, 100mg/Kg) in diabetic rats infected or not by Candida albicans (CA). Male Wistar rats were divided in: control; DM; CA; CA+ASc; CA+NPASc; DM+CA; DM+CA+ASc; and DM+CA+NPASc. Rats were daily treated for 21 days, when glycemic profile, ectonucleotidase (NTPDase and 5'-NT), adenosine deaminase (ADA), acetylcholinesterase (AChE) and dipeptidyl peptidase IV (DPP-IV) activities and nitric oxide (NO) and cytokine levels were analyzed in serum, platelets, lymphocytes and tissues. The results showed that NTPDase, 5'-NT and ADA activities and NO, IL-1, IL-6, TNF-α and IFN-É£ levels were increased in C. albicans, DM and DM+CA. The treatment with ASc and NPASc decreased ectonucleotidase and AChE activities and NO levels. Both treatments also prevented the increase in ADA activity and pro-inflammatory cytokines in cells and serum. In liver and pancreas, NPASc decreased NO levels more efficiently than ASc. The modulation of ectoenzyme activities can be one of the mechanisms by which S. cumini act on cytokines that affect the development of chronic complications in DM.


Keyword:     Diabetes model Candida albicans infection cytokines ectonucleotidases Syzygium cumini seeds nanotechnology.


Citation:

Bitencourt PER, Cargnelutti LO, Stein CS, Lautenchleger R, Ferreira LM, Sangoi M, Boligon A, Duarte MMMF, Moresco RN, Cruz L, Zanette RA, Alves SH, Moretto MA. Anti-inflammatory action of seed extract and polymeric nanoparticles of Syzygium cumini in diabetic rats infected with Candida albicans. J App Pharm Sci, 2017; 7 (01): 007-016.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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