Research Article | Volume: 6, Issue: 12, December, 2016

Evaluation of the anti-diarrheal activity of methanol extract and its fractions of Urena sinuata L. (Borss) leaves

Mir Muhammad Nasir Uddin Sumyya Zahan Md. Ashiqul Islam Shahriar Ahmed Tajbiha-E-Mowla Mohammad Sofiqur Rahman Ramiz Ahmed Sultan Talha Bin Emran   

Open Access   

Published:  Dec 28, 2016

DOI: 10.7324/JAPS.2016.601208

Objectives: Diarrhoeal disease is often a leading source of mortality and morbidity, especially among children in developing countries causing a major healthcare problem. As per suggestion of WHO, treatment of diarrhoea with traditional medicine is the main focus of our present studies. The present study is designed to evaluate the anti-diarrhoeal activity of crude methanol extract of Urena sinuata L. (U. sinuata) and its fractions.

Methods: Crude methanol extracts are obtained by in vacue methods and its fractionating is done by Kupchan partitioning method. The anti-diarrhoeal activity is screened by castor oil-induced diarrhoea, castor oil-induced enteropooling and gastrointestinal motility test.

Results: The diarrheal severity was reduced significantly (P < 0.01) by ethyl acetate fraction by 37.01% whereas 44.78% inhibition was found for standard drug loperamide at 5 mg/kg. The two fractions namely hydro methanol, ethyl acetate and crude methanol extract also significantly (P < 0.01) reduced the intestinal volume in case of castor oil induced enteropooling.

Conclusion: It is concluded that, leaves of U. sinuata contains bioactive natural substances with anti-diarrhoeal properties. These attributes may give a justification for your use of U. sinuata in diarrhoea management by traditional healers.

Keyword:     Anti-diarrheal activity Urena sinuata L. Castor oil Enteropooling Intestinal transit Solvent-solvent partitioning.


NasirUddin MM, Zahan S, Islam A, Ahmed S, Mowla TE, Rahman MS, Emran TB. Evaluation of the anti-diarrheal activity of methanol extract and its fractions of Urena sinuata L. (Borss) leaves. J App Pharm Sci, 2016; 6 (12): 056-060.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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