Research Article | Volume: 6, Issue: 11, November, 2016

Sub-chronic oral toxicity study of Kushta Hajrul-Yahood (A Herbo-mineral Unani formulation) in Wistar rats

Showkat A. Dar Seema Akbar Khalid Ghazanfar Mariya Hamdani Tazeen Nazir Masood S Mir Akbar Masood Humma Rafiq Showkat A Ganie   

Open Access   

Published:  Nov 29, 2016

DOI: 10.7324/JAPS.2016.601117

PD (Kushta Hajrul Yahood) is a Unani drug used for the treatment of renal and bladder calculi. The aim of the study was to demonstrate the scientific validity of detoxification method for preparation of Kushta and also to investigate the sub-chronic oral toxicity study of PD and CM (Crude material of Kushta Hajrul Yahood-its Un-detoxified form) in Wistar rats. Animals were orally treated once daily with test substance at the dose level of 1000mg and its two sub fractions 500 and 250mg/kg body weight for 90-days. Rats were observed throughout the study period. Body weight, feed and water consumption were recorded weekly. Overnight fasted rats were sacrificed on 91st day. Blood sample were collected for Haematological and biochemical studies. Organs were collected to record the organ weight and tissues for histology. The results showed elevation of liver enzymes due to the administration of PD at the dose level of 1000mg/kg body weight only, while-as in case of CM, there were significant elevation of liver enzymes at all the three doses. This elevation of enzymes also correlates with histopathological changes in liver tissues. This study concludes that the method of detoxification adopted for preparing Kushta substantially reduces the elements of toxicity.

Keyword:     Sub-chronic toxicity Hajrul-Yahood biochemical parameters haematological parameters Histopathology.


Dar S, Akbar S, Ghazanfar K, Hamdani M, Nazir T, Mir MS, Masood A, Rafiq H, Ganie SA. Sub-chronic oral toxicity study of Kushta Hajrul-Yahood (A Herbo-mineral Unani formulation) in Wistar rats. J App Pharm Sci, 2016; 6 (11): 105-113.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics

351 Absract views 11 PDF Downloads 362 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required