Research Article | Volume: 6, Issue: 10, October, 2016

Formulation and evaluation of folic acid conjugated gliadin nanoparticles of curcumin for targeting colon cancer cells

Shipra Sonekar Manoj Kumar Mishra Anil Kumar Patel Suresh Kumar Nair Chandra Shekhar Singh Amit Kumar Singh   

Open Access   

Published:  Oct 29, 2016

DOI: 10.7324/JAPS.2016.601009
Abstract

Preparation of folic acid (FA) conjugated (FA-CUR-GNPs) and non-conjugated (CUR-GNPs) gliadin nanoparticles of curcumin were successfully formulated by desolvation method for oral delivery of drug for targeting colon cancer cell. F1, F3, F5 (conjugated) and F2, F4, F6 (Non-conjugated) were formulated using various drug-polymer ratio (1:2). They were further characterized by FTIR, Mass spectroscopy, NMR, solubility studies, entrapment efficiency, TEM, particle size, surface charge, In-vitro release studies, In vivo toxicity studies and simultaneously evaluated. F3 (curcumin 10mg, gliadin 20mg and FA 5mg) and F4 (curcumin 10mg and gliadin 20 mg) were found as the optimized formulation among both the categories. For F3 and F4 formulations; average particle size (168.1 and 195.7nm), zeta potential (-16.5 and -24.4mV), cumulative % drug release (92.92 and 94%) and In vivo toxicity studies were conducted and compared with the control (phosphate-buffer saline, pH 6.8) reveals no toxicity. From the characterization and evaluation studies it was identified that F4 (FA-CUR-GNPs) had better solubility, In vitro release profile and no specified In-vivo toxicity than F3 (CUR-GNPs) formulation with nano-range particle size throughout the experiment. Improved bioavailability and increase targeting capacity toward colon cancer tumor cells were successfully achieved.


Keyword:     Chlorella sp. Synechocystis sp. nitrogen phosphorus waste water.


Citation:

Sonekar S, Mishra MK, Patel AK, Nair S, Singh CS, Singh AK. Formulation and evaluation of Folic acid conjugated gliadin nanoparticles of curcumin for targeting colon cancer cells. J App Pharm Sci, 2016; 6 (10): 068-074.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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