Research Article | Volume: 6, Issue: 8, August, 2016

Comparative study of chemical compositions and antimicrobial effect of different genius of Thymus harvested during two period of development

Aïcha Touhami Azzedine Chefrour Abbes Boukhari Fadhel Ismail   

Open Access   

Published:  Aug 30, 2016

DOI: 10.7324/JAPS.2016.60808
Abstract

The essential oils obtained by steam distillation of the aerial parts of Thymus taxa collected before and during the flowering period in different region of eastern Algeria, were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The extraction yields of essential oils of T. numidicus species are 1.28 % during pre-flowering. This yield is better during the flowering period with 2.85%. T. algeriensis gave extraction yields 1.156 % and 1.79 % in the two periods respectively. T. ciliatus produced oil with yields of 1.002 % before flowering and 1.79 % during flowering. Chromatographic analysis (GC-MS) showed that these essential oils are rich with oxygenated monoterpene compounds with 54.07 % and 80.37% of the composition for T. ciliatis, 61.86 % and 73.02 % for T. algeriensis, 54.48 % and 77.56 % for T. numidicus, corresponding to the two periods mentioned above respectively. The antimicrobial activity was tested against 3 gram positive and gram negative bacterial by the use of the disc diffusion method. The thymus taxa have shown great sensitivity. The three essential oils are effective against Escherichia. coli 22, while essential oil of T. ciliatus obtained before flowering and essential oil of T. numidicus in flowering period are most effective against Staphyllococcus 23.


Keyword:     T. numidicus T. ciliatus T. algeriensis GC-MS Antimicrobian activity.


Citation:

Touhami Aicha, Chefrour Azzedine, Boukhari Abbes, Ismail Fadhel. Comparative study of chemical compositions and antimicrobial effect of different genius of Thymus harvested during two period of development. J App Pharm Sci, 2016; 6 (08): 051- 056.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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