Research Article | Volume: 6, Issue: 7, July, 2016

Essential oil composition and variability of Artemisia herba-alba Asso. growing in Tunisia: comparison and chemometric investigation of different plant organs

Sana Bellilia b Wissal Dhific Abdulrahman Ben Khsif Al-Garnid Guido Flaminie Wissem Mnifa d   

Open Access   

Published:  Jul 28, 2016

DOI: 10.7324/JAPS.2016.60705

This study was conceived to investigate the composition of four essential oils (EOs) extracted by hydrodistillation from four parts (leaves, stems, leaves/stems, roots) of Artemisia herba-alba growing wild in the Center of Tunisia. For this, Artemisia herba-alba aerial and roots parts were shade dried with ventilation at room temperature. Then, plant different parts were cut into small pieces and subjected to hydrodistillation using a Clevenger-type apparatus. The gas chromatography (GC) analyses were accomplished with a HP-5890 Series II instrument. The main results showed a total of 152 compounds detected and identified by GC and GC-MS and accounting for 91.3-99.7% of the whole oil. The four oils were characterized by the predominance of monoterpene derivatives (68.2-99.5%) and the major volatile constituent was α-thujone (18.2-45.5%). Qualitative and quantitative differences between the four essential oils have been noted for some compounds. The main compounds of leaves essential oil were α-Thujone (45.5%), β-Thujone (11.4%), trans-sabinyl acetate (10.1%), 1,8-Cineole (7.4%) and camphor (6.8%). α-Thujone (27.5%) was also the main compound in the essential oil of leaves/stems, followed by camphor (22.9%), 1,8-cineole (8.3%), β-thujone (8.2%) and camphene (5.6%). The essential oil of stems was dominated by α-Thujone (28%) followed by β-Thujone (11.4%) and chrysantenone (11%). In the essential oil of roots, α-thujone was less represented (18.2%), followed by camphor (14.6%) and curcumen-15-al (14.3%). It is important to mention that curcumen-15-al has been reported for the first time in Artemisia herba-alba oil Our results revealed avariability in the chemical composition and the yield of the EOs from Artemisia herba-alba. Moreover, curcumen-15-al is a new chemotype first found in Artemisia herba-alba from Tunisia.

Keyword:     Artemisia herba-albaGC-MS chemical variability curcumen-15-al.


Bellili S, Dhifi W, Al-Garni ABK, Flamini G, Mnif W. Essential oil composition and variability of Artemisia herba-alba Asso. growing in Tunisia, comparison and chemometric investigation of different plant organs. J App Pharm Sci, 2016; 6 (07): 038-042.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics
361 Views 31 Downloads 392 Total



Related Search

By author names