Research Article | Volume: 6, Issue: 4, April, 2016

GABAergic and nitriergic influence in antianxiety-like Activity of Garlic in Mice

Neeraj Gilhotra Dinesh Dhingra   

Open Access   

Published:  Apr 30, 2016

DOI: 10.7324/JAPS.2016.60411

Objectives: To investigate the GABAergic and nitriergic mechanism involved in the anxiolytic-like profile of ethanolic extract of garlic (GE).

Materials and Methods: Male Swiss albino mice were employed in the present study. Stress was produced in mice by immobilizing them for 6h. Elevated plus maze, light/dark box and social interaction test were used for the assessment of anxiety in mice. Concentrations of GABA in brain and nitrite level in plasma were estimated to determine the possible involvement of GABAergic and nitriergic mechanisms in the anxiolytic profile of GE.

Results: The present study showed that the GE produced significant antianxiety- like activity in unstressed and stressed mice. In unstressed mice, GE significantly increased GABA levels, but could not produce any change in nitrite levels. Meanwhile, in stressed mice, GE significantly increased GABA levels along with a significant decrease in nitrite levels. Pre-treatment with aminoguanidine, an inducible nitric oxide synthase inhibitor, significantly enhanced the anxiolytic-like activity of GE, as compared to GE and aminoguanidine alone in stressed mice, but not in unstressed mice. On the other hand, pretreatment with 7-nitroindazole, a neuronal nitric oxide synthase inhibitor, did not produce any significant change in antianxiety- like activity of GE in unstressed as well as stressed mice.

Conclusion: It has been concluded that the garlic may possess anxiolytic- like activity and possess NOS inhibiting property in stressed mice, which may add to its status to be used in stress-induced anxiety conditions.

Keyword:     Aminoguanidine anxiety GABA garlic nitric oxide.


Neeraj Gilhotra, Dinesh Dhingra. GABAergic and nitriergic influence in antianxiety-like Activity of Garlic in Mice. J App Pharm Sci, 2016; 6 (04): 077-085.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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