Research Article | Volume: 6, Issue: 2, February, 2016

Determination of antidiabetic compounds from Helicteres isora fruits by oral glucose tolerance test

Govindasami Chandirasegaran Chakkaravarthy Elanchezhiyan Kavisa Ghosh Subramaniyan Sethupathy   

Open Access   

Published:  Feb 27, 2016

DOI: 10.7324/JAPS.2016.60227

The aim of this study was to analyze compounds from the methanolic extract of fruits of Helicteres isora for antidiabetic activity. Sanguinarine, berberine chloride (BC) and muscimol were found to be as major compounds in the methanolic fruits extract of H. isora. BC was isolated from the methanolic fruit extract of H. isora by using HPLC and other compounds were commercially acquired. Diabetes was induced in rats by a single dose of intraperitonial injection of streptozotocin (STZ). Sanguinarine (50 mg/kg b.w), BC (50 mg/kg b.w) and muscimol (50 mg/kg b.w) were evaluated by Oral Glucose Tolerance Test (OGTT) in normal and STZ induced diabetic rats. Among the three compounds, BC significantly reduced the blood glucose levels when compared to sanguinarine and muscimol. The OGTT study clearly indicates, BC possess promising antidiabetic activity against STZ induced diabetic rats.

Keyword:     Helicteres isora Diabetes Sanguinarine Berberine chloride and Muscimol.


Govindasami C, Chakkaravarthy E, Ghosh K. Determination of antidiabetic compound from Helicteres isora by oral glucose tolerance test. J App Pharm Sci, 2016; 6 (02): 172-174.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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