Research Article | Volume: 6, Issue: 1, January, 2016

Antimicrobial activities of mycoleptodiscin B isolated from endophytic fungus Mycoleptodiscus sp. of Calamus thwaitesii Becc.

Ranga K. Dissanayake Pamoda B. Ratnaweera David E. Williams C. Dilrukshi Wijayarathne Ravi L. C. Wijesundera Raymond J. Andersen E. Dilip de Silva   

Open Access   

Published:  Jan 26, 2016

DOI: 10.7324/JAPS.2016.600101
Abstract

Endophytic fungi are a promising source of novel biologically active compounds including antimicrobials. Plant endophytic fungi of Sri Lanka, an island with exceptionally high biodiversity and endemism, are a vastly untapped resource. Therefore this study was initiated with the objective of examining the antimicrobial producing potential of the endophytic fungi of Calamus thwaitesii Becc. from Sri Lanka. This examination resulted in the isolation of 21 fungi with 7 of them exhibiting antimicrobial properties. Further investigation of the Mycoleptodiscus sp. isolated from the leaves, which showed the best activity among them, resulted in the isolation of the known alkaloid mycoleptodiscin B and its structure was elucidated and confirmed by mass and nuclear magnetic resonance spectral data. Mycoleptodiscin B showed promising antimicrobial activity against Bacillus subtilis (MIC 0.5 µg mL-1) and Staphylococcus aureus (MIC 1 µg mL-1), and was less potent against methicillin resistant Staphylococcus aureus (MRSA, MIC 32 µg mL-1) and the pathogenic fungus Candida albicans (MIC 64 µg mL-1). This is the first study to report the isolation, identification and antimicrobial properties of endophytic fungi of C. thwaitesii and the antimicrobial activities of the alkaloid mycoleptodiscin B.


Keyword:     Mycoleptodiscus sp. Calamus thwaitesii mycoleptodiscin B endophytic fungus antimicrobial.


Citation:

Dissanayake RK, Ratnaweera PB, Williams DE, Wijayarathne CD, Wijesundera RLC, Andersen RJ, deSilva ED. Antimicrobial activities of mycoleptodiscin B isolated from endophytic fungus Mycoleptodiscus sp. of Calamus thwaitesii Becc. J App Pharm Sci, 2016; 6 (01): 001-006.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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