Research Article | Volume: 5, Issue: 12, December, 2015

Proteolytic and Milk Clotting Activity of Fractionated Protein of Lagenaria siceraria

Priyanka Dash Laxmidhar Maharana Goutam Ghosh   

Open Access   

Published:  Dec 27, 2015

DOI: 10.7324/JAPS.2015.501214

The study was undertaken to evaluate milk clotting and proteolytic activity of protein fractions of seeds of Lagenaria siceraria. Protein fractions were isolated by the method of differential solubility, and their protein contents were estimated using Bio-Rad protein assay reagent and bovine serum albumin. The effects of pH and temperature on the milk-clotting and proteolytic activity were also evaluated. The isolated protein fractions showed highest milk clotting activity over a broad temperature range of 30-80 ºC and pH range of 3-9. Based upon the observations, milk clotting enzymes present in different fraction of proteins of Lagenaria siceraria are promising candidates for application in industrial scale for production of cheese and might be a potential substitute for commercial animal rennet.

Keyword:     Proteolytic milk clotting Lagenaria siceraria.


Dash P, Maharana L, Ghosh G. Proteolytic and Milk Clotting Activity of Fractionated Protein of Lagenaria siceraria. J App Pharm Sci, 2015; 5 (12): 086-091.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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