Research Article | Volume: 5, Issue: 12, December, 2015

Preliminary in vitro antimicrobial screening of chemical constituents isolated from the root of Lepleae mayombensis (Meliaceae)

Lazare Sidjui Sidjuia b c,  Yvan Anderson Ngandjui Tchangoued,  Sindhu Radhakrishnanc,  Perumal Karthigae,  Paul Djomgoueh,  Rufin Marie Kouipou Toghueof,  Louis Claire Ndel Fameng,  Gurumasy Annaduraic,  Gabriel Ngosong Folefoc   
Lazare Sidjui Sidjuia & b & c, Yvan Anderson Ngandjui Tchangoued, Sindhu Radhakrishnanc, Perumal Karthigae, Paul Djomgoueh, Rufin Marie Kouipou Toghueof, Louis Claire Ndel Fameng, Gurumasy Annaduraic, Gabriel Ngosong Folefoc

aInstitute of Medical Research and Medicinal Plants Studies P.O Box 6163 Yaoundé, Cameroon.

bDepartment of Organic Chemistry, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon.

cSri Paramakalyani Centre of Excellence in Enviromental Sciences Manonmaniam Sundaranar University, Alwarkurichi -627412, Tamil Nadu, India.

dDepartment of chemistry, Higher Teachers Training College, P.O Box 47, Yaoundé, Cameroon.

eDepartment of Chemistry, Manonmaniam Sudaranar University, Tirunelveli - 627012, Tamil Nadu, India.

fDepartment of Biochemistry, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon.

gDepartment of Pharmacy, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O Box: 2701 Douala, Cameroon.

hTU Chemnitz, Institut Für Anorganische Chemie, Straße Der Nationen 62 0911 Chemnitz, Germany.

Open Access   

Published:  Dec 27, 2015

DOI: 10.7324/JAPS.2015.501206
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Abstract

The Phytochemical study of Methanol/dichloromethane extract of root of Leplaea mayombenis led to the isolation and identification four limonoids: (8S,9R,10R,11S,13R,17R)-6,11-dihydroxy-14,15-epoxy-4,4,8,10,13-pentamethyl-17-(2-oxo-2,5-dihydrofuran-3-yl-)-11,12,13,15,16,17-hexahydro-4H-cyclopent[α] phenanthrene-3,7(8H,9H,10H,14H)-dione (1), (8S,9R,10R,11S,13R,17R)-6,11-dihydroxy-14,15-epoxy-4,4,8,10,13-pentamethyl -17-(23-methoxy-2-oxo-2,5-dihydrofuran-3-yl-)-11,12,13,15,16,17-hexahydro-4H-cyclopent [α] phenanthrene 3,7(8H,9H,10H,14H)-dione (2), (8S,9R,10R,11S,13R,17R)-6,11-dihydroxy-14,15-epoxy-17- ((s)-2-hydroxy-5-oxo-2,5-dihydrofurano-3-yl)-4,4,8,10,13-pentamethy-l,10,11,12,13,15,16,17-octahydro-4H-cyclopent [α] phenanthrene-3,7(8H,9H,10H,14H)-dione (3), 8S,9R,10R,11S,13R,17R)-1,6,11-trihydroxy-14,15-epoxy-17- [((2s)-2-hydroxy-5-oxo-2,5-dihydrofurano-3-yl)]-4,4,8,10,13-pentamethyl-l,10,11,12,13,15,16,17-octahydro-4H-cyclopent[α]phenanthrene-3,7(8H,9H,10H,14H)-dione (4), one cycloartane : 9,10-cyclopropyl-(3S,5R,8R,13R,14S,17R))-4,4,13,14,17-pentamethyl-l7-((2R,5S,6R)-5,6,7-trihydroxy-6-methylheptan-2-yl)-hexadecahydro-1H-cyclopentane[α]phenanthrèn-3-ol (5) and three steroids: β-sitosterol (6), stigmasterol (7) and stigmasterol 3-O-β-D-glucopyranoside (8). Their structures were elucidated on the basis of spectroscopic analysis and by comparison of their spectral data with those reported in the literature. To the best of our knowledge, all these compounds were isolated for the first time from the Leplaea mayombensis. The antimicrobial studies showed that isolated compounds exhibit antimicrobial activity with inhibition zone diameters varying from 0.00 ± 0.00 to 29.00 ± 0.00 mm on both bacteria and fungi. 9,10-cyclopropyl-(3S,5R,8R,13R,14S,17R) )-4,4,13,14,17-pentamethyl-l7-((2R,5S,6R)-5,6,7-trihydroxy-6-methylheptan-2-yl)-hexadecahydro-1 H-cyclopentane[α] phenanthrèn-3-ol (5) was the most active against Escherichia coli (28.00±0.00 mm), Pseudomonas sp (27.00±0.00 mm), Lactobacillus acidophilus (29.00±0.00 mm), Streptococcus pneumonia (23.00±0.00 mm) and Serratia entomophili (29.00±0.00 mm), C. albicans (12±0.00 mm), and T. viridae (13±0.00 mm) The results from this study support the conclusion that L. mayombensis contain many classes of antimicrobial compounds and therefore justify their traditional usage in the treatment of infectious diseases.


Keyword:     Leplaea mayombensisPhytochemical studyLimonoidsCycloartaneantimicrobial.

Citation:

Sidjui LS, Tchangoue YAN, Radhakrishnan S, Karthiga P, Toghueo RMK, Famen LCN, Djomgoué P, Annadurai G, Folefoc GN. Preliminary in vitro antimicrobial screening of chemical constituents isolated from the root of Lepleae mayombensis (Meliaceae). J App Pharm Sci, 2015; 5 (12): 035-041.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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