Research Article | Volume: 5, Issue: 10, October, 2015

Isolation and Characterization of a Lactose-Binding Lectin from Ocimum sanctum

Praveen Kumar Vemuri Bhavana Talluri Ananya Sharma Geethika Akkala Vijaya Lakshmi Bodiga   

Open Access   

Published:  Oct 28, 2015

DOI: 10.7324/JAPS.2015.501019
Abstract

A novel lectin has been isolated from Ocimum sanctum and purified to homogeneity by gel filtration chromatography, which eluted as a single symmetrical peak from a Biogel P-100 column with a molecular mass of 66 kDa. The lectin had a special agglutinating activity with human erythrocytes at a minimum concentration of 0.7 ug/ml. The lectin was stable in the pH range 5-12 and temperature 80 °C for 30 min. Ocimum sanctum had shown highest agglutinating activity at pH7 and 250C temperature after one hour incubation. Of the various sugars tested, even at 1000 mM sugar concentration, no inhibition was observed. The isolated lectin was found to be lactose-biding lectin sugar moieties and contain 2.6 mg/ml total sugar and 9.3 mg/ml of total protein.


Keyword:     Lectin Blood group Glycobiology ELISA Agglutination Ocimum sanctum.


Citation:

Praveen Kumar Vemuri, Bhavana Talluri, Ananya Sharma, Geethika Akkala, Vijaya Lakshmi Bodiga. Isolation and Characterization of a Lactose-Binding Lectin from Ocimum sanctum. J App Pharm Sci, 2015; 5 (10): 113-117.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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