Research Article | Volume: 5, Issue: 9, September, 2015

Polyphenolic contents of some instant tea brands and their anti-oxidant activities

Patricia Odumosu Stephen Ojerinde Myrrh Egbuchiem   

Open Access   

Published:  Sep 27, 2015

DOI: 10.7324/JAPS.2015.50918
Abstract

Tea which is one of the most widely consumed beverages after water, contains diverse polyphenols such as flavonoids and other catechins. These polyphenols confer anti-oxidant properties on tea with the capacity to slow down or prevent oxidation of other molecules. Ten instant tea brands were tested for purity and anti-oxidant properties. Qualitative tests for anti-oxidant activities were visualized by thin layer chromatography (TLC) and DPPH (2, 2-diphenyl-1-picrylhydrazyl) spray. The extent of purity was determined by tests for extractable matter, ash values and moisture content whereas total polyphenolic content was determined by the colorimetric method using Folin-Ciocalteu reagent. Antioxidant activity was determined by DPPH free radical scavenging assay using rutin, vitamin C and gallic acid as standards. The test with TLC-DPPH spray showed that all ten brands had anti-oxidant components and the tea with the highest total phenolic content had a value of 360.62 ± 31.47mg GAE/g whereas the lowest was 150.76 ± 23.13mg GAE/g in contrast to 33.24 ± 15.89 and 6.47 ± 1.20mg RE/g for flavonoid content. The tea (black tea) with the most potent anti-oxidant activity had a potency value of 5.90 while the least potent (green tea) had a value of 4.20.The results are suggestive of good antioxidant properties of instant tea.


Keyword:     Instant tea DPPH anti-oxidant assay polyphenol and flavonoid contents radical scavenging.


Citation:

Patricia Odumosu, Stephen Ojerinde, Myrrh Egbuchiem. Polyphenolic contents of some instant tea brands and their antioxidant activities. J App Pharm Sci, 2015; 5 (09): 100-105.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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