Research Article | Volume: 5, Issue: 9, September, 2015

Antimicrobial activity and chemical composition of essential oil and hydrosol extract of Nepeta nepetella subsp. amethystina (Poir.) Briq. from Algeria

Chafika Bellahsene Mourad Bendahou Abdelmounaïm Khadir Fatima Zenati Fethi Benbelaïd Nadia Aissaoui Alain Muselli Jean Costa   

Open Access   

Published:  Sep 27, 2015

DOI: 10.7324/JAPS.2015.50904
Abstract

The essential oil and hydrosol extract of Nepeta nepetella subsp. amethystina, obtained by hydrodistillation and analysed by GC, GC–MS, 1H and 13C NMR, were evaluated for their antimicrobial activity using disks diffusion method and MICs against thirteen pathogenic microorganisms. The chemical analysis of essential oil and hydrosol extract allowed the identification of 34 and 12 constituents representing 96.77% and 80.07% of total oil and hydrosol extract, respectively. The two isomers 4aα,7α,7aβ-nepetalactone and 4aα, 7α, 7aα-nepetalactone were found to be the major components in N. amethystina essential oil and hydrosol extract, α, 7α-nepetalactone and 4aβ,7aα,7α respectively. The two isomers 4a -nepetalactone were found to be the major components in N.amethystina essential oil and hydrosol extract. Candida albicans strains were the most sensitive microorganisms towards N. amethystina essential oil and hydrosol extract, which have the largest inhibition zones (˃30mm) and the lower MICs values (<1.25%). The anti-mold activity, carried out by the diffusion method, showed that N. amethystina essential oil has a very interesting activity against Aspergillus flavus and Cladosporium herbarium.


Keyword:     Nepeta nepetella subsp. amethystinaessential oilhydrosol extractnepetalactonesantimicrobial activity.


Citation:

Chafika Bellahsene, Mourad Bendahou, Abdelmounaïm Khadir, Fatima Zenati, Fethi Benbelaid, Nadia Aissaoui, Alain Muselli, Jean Costa. Antimicrobial activity and chemical composition of essential oil and hydrosol extract of Nepeta nepetella subsp. amethystina (Poir.) Briq. from Algeria. J App Pharm Sci, 2015; 5 (09): 021-025.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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