Research Article | Volume: 5, Supplement: 2, September, 2015

Trichoderma asperellum isolated from salinity soil using rice straw waste as biocontrol agent for cowpea plant pathogens

Eman R. Hamed Hassan M. Awad Eman A. Ghazi Nadia G. El-Gamal Heba S. Shehata   

Open Access   

Published:  Sep 04, 2015

DOI: 10.7324/JAPS.2015.58.S15

This study discusses isolation and identification new fungal isolate from salinity soil for controlling soil borne diseases. Among sixteen fungal, a potent isolate coded SRBP_ZSHSG1 was isolated from Sugar beet rhizospher samples collected from Al-Hosainia localities-El-Sharkia-Egypt. Traditional methods consistent with phylogenetic analysis of 18S rRNA sequences showed SRBP_ZSHSG1 has 100% similarity with Trichoderma strains and the most closest is Trichoderma asperellum. Thus, it proposed name Trichoderma asperellum SRBP_ZSHSG1 (ID: KP336489). Results proved SRBP_ZSHSG1 followed by T. roseum and Chaetomium globosum were highly inhibitors to the tested pathogens. These results were confirmed by field experiments. SRBP_ZSHSG1 was able to grow on rice straw (biostraw) and produce most active compounds. The biostaw extract was the most effective bioagent and recorded highest reduction in pathogen numbers. GC/MS analysis of ethyl acetate extract revealed the presence of 9 compounds. These compounds were determined 4 volatile alcohols (1-4) and fatty acid esters (5-9).

Keyword:     Trichoderma sp.phenotypic and genotypic identificationRoot rotWiltcowpea plant.


Eman R Hamed, Hassan M Awad, Eman A Ghazi, Nadia G ElGamal, Heba S Shehata. Trichoderma sp. isolated from salinity soil using rice straw waste as biocontrol agent for cowpea plant pathogens. J App Pharm Sci, 2015; 5 (Suppl 2): 091-098.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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