Research Article | Volume: 5, Issue: 8, August, 2015

Antimicrobial activity of compounds from Acanthospermum hispidum DC and Caesalpinia bonduc (L.) ROXB: Beninese plants used by healers against HIV-associated microbial infections

Alban G. Houngbèmè,  Habib M.Y.Ganfon,  Sédami Medegan ,  ,  Boniface Yèhouénou,  Bouraïma Bambola,  Clément Gandonou,  Fernand A. Gbaguidi ,    
Alban G. Houngbèmè1, Habib M.Y.Ganfon1, Sédami Medegan1 , 2, Boniface Yèhouénou3, Bouraïma Bambola1, Clément Gandonou1, Fernand A. Gbaguidi1 , 2

1Laboratoire de Pharmacognosie/Institut de Recherche et d’Expérimentation en Médecine et Pharmacopée Traditionnelles (IREMPT) / Centre Béninois de la Recherche Scientifique et Technique (CBRST)/ UAC, 01 BP 06 Oganla Porto-Novo.

2Laboratoire de Chimie Pharmaceutique Organique, Ecole de Pharmacie, Faculté des Sciences de la Santé, Université d'Abomey-Calavi, Campus du Champ de Foire, 01 BP 188, Cotonou, Bénin.

3Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA)/Ecole Polytechnique d’Abomey-Calavi/Université d’Abomey-Calavi, 01 BP 2009 Cotonou.

Open Access   

Published:  Aug 28, 2015

DOI: 10.7324/JAPS.2015.50812
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Abstract

In this study, we have tested alcoholic extracts (60%) from four Beninese plants: Ocimum gratissimum L., Acanthospermum hispidum DC, Caesalpinia bonduc (L) Roxb and Calotropis procera W. T. Aiton. They are used by the healers to prevent opportunistic diseases associated to HIV-AIDS; on six strains such as: Escherichia coli O 157H7, Staphylococcus aureus ATCC 25923 which resist to methicillin (MRSA), Salmonella typhi, Klebsiella pneumonia, Candida albicans ATCC 10231, and Mycobacterium bovis BCG 040812 which cause microbial infections associated with HIV-AIDS. The results show that all the extracts are bacteriostatic and fungistatic but only the hydro-ethanolic extracts of Acanthospermum hispidum (HE2) and of Caesalpinia bonduc (HE3) presented antibiotic power (respectively ap = 2 and ap = 4) on Candida albicans ATCC 10231. The Mycobacterium bovis BCG shown resistance to tested extracts (CMI > 250 µg/mL). The two fungicidal extracts HE2 and HE3 did not show harmful effects on the cells WI–38 with an IC50 > 100 µg/mL for HE2 and IC50 = 50 µg/mL for HE3. The successive bio-guided purifications of extracts HE2 and HE3 permitted isolation of three antibacterial compounds: Flavanone (M1); stigmasterol (M2); and quercetin (M3). The three isolated compounds possess antibiotic power (ap 3±1) on tested strains and are not toxic on shrimp larvae (LC50: 0.30 ± 0.17 mg/mL).


Keyword:     Microbial infections HIV-AIDS Beninese plants cytotoxic activity antimicrobial activity chromatographic methods.

Citation:

Alban G. Houngbèmè Habib M.Y.Ganfon, Sédami Medegan, Boniface Yèhouénou, Bouraïma Bambola, Clément Gandonou, and Fernand A. Gbaguidi. Antimicrobial activity of compounds from Acanthospermum hispidum DC and Caesalpinia bonduc (L.) ROXB: Beninese plants used by healers against HIV-associated microbial infections. J App Pharm Sci, 2015; 5 (08): 073-081.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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