Published:  Jul 27, 2015DOI: 10.7324/JAPS.2015.50716
An efficient low cost procedure for in vitro propagation of Chrysanthemum morifolium has been developed with subsequent assessment of antibacterial property of in vitro raised plantlets. Optimal axillary shoot multiplication was achieved on MS medium supplemented with low concentration of BAP. Psyillium husk and market sugar were standardized as suitable alternatives to the conventionally used agar and sucrose, cutting down the production cost of tissue culture raised plantlets to over six times. Optimal in vitro rooting was obtained on half strength MS medium containing IBA. Regenerated plantlets with well developed shoots and roots were acclimatized successfully and transferred to field conditions where they flowered. The leaves of ex vitro growing tissue culture raised plantlets were later assessed for activity against bacterial pathogens. The present protocol ensures minimal cost input in large scale production of a commercially important ornamental plant and opens up scope of scientific interventions directed at its allied therapeutic usage.
Manu Pant, Ankita Lal, Rashi Jain. A simple cost effective method for mass propagation of Chrysanthemum morifolium and antibacterial activity assessment of in vitro raised plantlets. J App Pharm Sci, 2015; 5 (07): 103-111.
339 Absract views 17 PDF Downloads 356 Total views
Current Situation of Availability and CostEffectiveness Analysis of Selected Drugs inAnantapur, AP, India
Prodigiosin - an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria