Research Article | Volume: 5, Issue: 5, May, 2015

Enzymatic and Non Enzymatic Antioxidant Activity of Tabernaemontana divaricate R.Br. against DEN and Fe-NTA Induced Renal Damage in Wistar Albino Rats

Sowmya Sundaram Gopalakrishnan Velliyur Kanniappan Poornima Kannappan   

Open Access   

Published:  May 27, 2015

DOI: 10.7324/JAPS.2015.50506
Abstract

Plant derived drugs have been a part of the evolution of human healthcare for thousands of years. The present study was carried out to evaluate the antioxidant potential of ethanolic extract of Tabernaemontana divaricata on DEN initiated and Fe-NTA promoted renal damage in rats. Fe-NTA was induced after 10 days of DEN (200mg/kg body weight) initiation at a dose level of 9mg Fe-NTA/kg body weight twice a week for one month. The biochemical parameters were analyzed in serum and the antioxidant assays were carried out in kidney. Lipid peroxidation level was increased due to the administration of Fe-NTA, which caused the reduction of enzymatic antioxidant such as SOD, GPx, Catalase, G6PD, and also the non-enzymatic antioxidants vitamin C and GSH. The levels of urea, uric acid, creatinine, blood urea nitrogen were increased and protein level decreased on Fe-NTA intoxication. The secondary metabolites present in the plant increased the synthesis of antioxidant enzymes and its free radical scavenging properties helped to scavenge all free radicals thereby decreasing lipid peroxidation. Thus, the present study indicates that the plant may clinically valuable agent in the prevention of renal failure caused by DEN and Fe-NTA intoxication.


Keyword:     Tabernaemontana divaricata Diethyl nitrosamine Ferric nitrilotriacetate Medicinal plants Renal damage.


Citation:

Sowmya .S, Gopalakrishnan V.K and Poornima .K. Enzymatic and Non Enzymatic Antioxidant Activity of Tabernaemontana divaricate R.Br. Against DEN and Fe-NTA Induced Renal Damage in Wistar Albino Rats. J App Pharm Sci, 2015; 5 (05): 033-037.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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