Research Article | Volume: 5, Issue: 3, March, 2015

Antimicrobial and cytotoxic acetogenin from Polyalthia debilis

Somchai Boonpangrak,  Rungrot Cherdtrakulkiat,  Ratchanok Pingaew,  Patumporn Manam,  Supaluk Prachayasittikul,  Somsak Ruchirawat ,  Virapong Prachayasittikul   
Somchai Boonpangrak1, Rungrot Cherdtrakulkiat2, Ratchanok Pingaew3, Patumporn Manam3, Supaluk Prachayasittikul4, Somsak Ruchirawat5 6, Virapong Prachayasittikul2

1Center for Innovation Development and Technology Transfer, Faculty of Medical Technology,Mahidol University, Bangkok 10700, Thailand.

2Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

3Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

4Center of Data Mining and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

5Laboratory of Medicinal Chemistry, Chulabhorn Research Institute and Program in Chemical Biology, Chulabhorn Graduate Institute, Bangkok 10210, Thailand.

6Center of Excellence on Environmental Health and Toxicology, Commission on Higher Education (CHE), Ministry of Education, Thailand.

Open Access   

Published:  Mar 28, 2015

DOI: 10.7324/JAPS.2015.50303
PDF [ 0.2 MB]
Request Permission
Share
Download Citation
Print
Download PDF
Abstract

The objective of the study was to isolate bioactive acetogenin compound and to investigate antimicrobial, antioxidant as well as cytotoxic activities of the isolate, fractions and extracts of Polyalthia debilis. The P. debilis (roots) extracts and isolated compound were tested for their antimicrobial (agar dilution method) against twenty-seven strains of microorganisms (gram positive and gram negative bacteria, and diploid fungus), antioxidant (DPPH assay) and cytotoxic activities. The plant extracts were isolated by column chromatography and structure of compound was confirmed by spectral data. The plant extracts and isolated fractions exhibited antioxidant and cytotoxic activities. The isolated acetogenin 1 (debilisone E) displayed antimicrobial activity against Morexella catarrhalis with the MIC of 64 µg/mL, Corynebacterium diphtheriae NCTC 10356 and Streptococcus pyogenes with partial inhibition (50-75%) at 128 µg/mL. The compound 1 exerted cytotoxic activity against 5 cancer cells (HepG2, A549, HCC-S102,HL-60 and P388) with IC50 values 18.4 - 40.3µg/mL. The results demonstrate novel bioactivities of P. debilis as antimicrobials and anticancer agents.


Keyword:     Polyalthia debilis Annonaceae Bioactive acetogenin Antimicrobials Antioxidants Cytotoxic agents.

Citation:

Somchai Boonpangrak, Rungrot Cherdtrakulkiat, Ratchanok Pingaew, Patumporn Manum, Supaluk Prachayasittikul, Somsak Ruchirawat, Virapong Prachayasittikul. Antimicrobial and cytotoxic acetogenin from Polyalthia debilis. J App Pharm Sci, 2015; 5 (03): 013-018.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HTML Full Text

Reference

Article Metrics
382 Views 20 Downloads 402 Total

Year

Month

Related Search

By author names