Research Article | Volume: 5, Issue: 1, January, 2015

Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae)

Ahlem Rebaya,  Souad Igueld Belghith,  Béatrice Baghdikian,  Valérie Mahiou Leddet,  Fathi Mabrouki,  Evelyne Olivier,  Jamila kalthoum Cherif ,  Malika Trabelsi Ayadi   
Ahlem Rebaya1, Souad Igueld Belghith2, Béatrice Baghdikian3, Valérie Mahiou Leddet3, Fathi Mabrouki3, Evelyne Olivier3, Jamila kalthoum Cherif1 & 4, Malika Trabelsi Ayadi

1Laboratory of Applications of Chemical Resources, Natural Substances and the Environment (LACReSNE), Faculty of Sciences of Bizerte, 7021 Zarzouna - Bizerte, Tunisia.

2Preparatory Institute for Engineering Studies of El-Manar B.P.244 El Manar II - 2092 Tunis, Tunisia.

3Laboratory of Pharmacognosy and Ethnopharmacology, UMR-MD3, Faculty of Pharmacy, Aix-Marseille University, 27 Bd Jean Moulin, CS 30064, 13385, Marseille cedex 5, France.

4Preparatory Institute for Engineering Studies of Tunis, 2 rue Jawaharlal Nehru, Monfleury, 1008 Tunis, Tunisia.

Open Access   

Published:  Jan 30, 2015

DOI: 10.7324/JAPS.2015.50110
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Abstract

This study was carried out to evaluate the phytochemical constituents and antioxidant potential of the leaves and flowers extracts of Halimium halimifolium in order to validate the medicinal potential of this herb. The antioxidant activity of alcoholic and aqueous extracts was evaluated using 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2’-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. The total polyphenol, flavonoid and tannin content were determined according respectively to Folin-Ciocalteu method, Zhishen method and Broadhurst method. The leaves of H. halimifolium had greater antioxidant activity than flowers by DPPH and ABTS assays. The ethanol extract of the leaves exhibited the better antioxidant activity by DPPH assay (IC50 = 1.19 µg.mL-1), the n-butanol by ABTS assays (IC50 = 1.73µg.mL-1). As opposed to FRAP method the flowers had greater antioxidant activity as leaves. Ethyl acetate extract exhibited better antioxidant activity (IC50 = 15.5 mmol Fe2+.g-1). The obtained results showed that the extracting solvent significantly influenced the antioxidant property estimations of Halimium halimifolium. The ethanol is a recommended solvent for extracting antioxidants from this plant. The antioxidant activity determined by DPPH, ABTS and FRAP demonstrated a linear relationship with their polyphenols, flavonoids and tannins content.


Keyword:     Halimium halimifolium Cistaceae antioxidant activity polyphenols.

Citation:

Ahlem Rebaya, Souad Igueld Belghith, Béatrice Baghdikian, Valérie Mahiou Leddet, Fathi Mabrouki, Evelyne Olivier, Jamila kalthoum Cherif and Malika Trabelsi Ayadi. Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae). J App Pharm Sci, 2015; 1 (01): 052-057.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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