Research Article | Volume: 4, Issue: 12, December, 2014

Batch production and media optimization of anti-leukemic L-asparaginase from Pseudomonas fluorescens by Taguchi DOE methodology

Santosh Kumar Jha Divya Pasrija Hare Ram Singh Vinod Kumar Nigam Ambrish Sharan Vidyarthi   

Open Access   

Published:  Dec 29, 2014

DOI: 10.7324/JAPS.2014.41215

L-Asparaginase isolated from bacterial sources has been shown to possess antileukemic characteristic, mainly against the acute lymphoblastic leukemia. Large number of bacterial strains have been reported which can produce it. The production level of the enzyme by the bacterial sources is very low and hence the factors have to be distinguished which effect the growth of cells and production of enzyme directly or indirectly, in order to decrease the cost of treatment. The production of L-Asparaginase was optimized in classical fermentation process by the use of Taguchi DOE methodology. L-18 array was selected for the purpose of media optimization. The five factors at three levels were considered for the optimization. L-Asparaginase production was significantly (p<0.05) affected by the interaction of two factors present in the culture broth. The factors interaction viz. energy source-nitrogen source, energy source-phosphate source etc. having pronounced effect on the production while individually they have the minimum impact on L-Asparaginase production. So the interactions of different parameters were studied and they were used to formulate the optimized condition for the production of L-Asparaginase. After the validation of result by performing the experiment under optimized condition there was about 28.48% increase in the production of the enzyme.

Keyword:     L-Asparaginase Acute Lymphoblastic Leukemia Taguchi DOE Methodology.


Santosh Kumar Jha, Divya Pasrija, Hare Ram Singh, Vinod Kumar Nigam, Ambrish Sharan Vidyarthi. Batch Production and Media Optimization of Anti-leukemic L-Asparaginase from Pseudomonas Fluorescens by Taguchi DOE Methodology. J App Pharm Sci, 2014; 4 (12): 085-089.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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