Research Article | Volume: 4, Issue: 12, December, 2014

Combination of Cisplatin and Cinnamon Essential Oil Inhibits HeLa Cells Proliferation through Cell Cycle Arrest

Yonika Arum Larasati Dyaningtyas Dewi Pamungkas Putri Rohmad Yudi Utomo Adam Hermawan Edy Meiyanto   

Open Access   

Published:  Dec 29, 2014

DOI: 10.7324/JAPS.2014.41203
Abstract

Cisplatin is drug of choice toward cervical cancer despite having many side effects, thus researches are conducted in order to find the effective and synergistic co-chemoterapeutic agent combined with cisplatin. In this study, we observed the potential of the cinnamon essential oil (CEO) isolated from Cinnamomum burmannii as co-chemoterapeutic agent of cisplatin on HeLa cells covering cytotoxic effect, cell cycle modulation and induction of apoptosis. Cytotoxic effect was determined by using MTT assay; while induction of apoptosis and cell cycle profile were observed by using flow cytometry. At 24 hours of incubation, CEO showed cytotoxic effect on HeLa cells with IC50 value of 250 μg/mL, while cisplatin showed cytotoxic effect with IC50 value of 18 µM. Combination of CEO and cisplatin reduced cells viability compared to cisplatin solely. Moreover, flow cytometry using annexin-V and PI showed that CEO and its combination with cisplatin induced apoptosis lower than cisplatin alone at 24 hours of incubation. Further analysis on the cell cycle progression showed that CEO induced S-phase arrest on HeLa cells, cisplatin induced G1 arrest, while combination of CEO and cisplatin induced G2/M arrest. Thus, the inhibition of HeLa cells growth at 24 hours is likely through cell cycle modulation rather than apoptosis.


Keyword:     Cinnamon essential oil cisplatin HeLa cells apoptosis cell cycle.


Citation:

Yonika Arum Larasati, Dyaningtyas Dewi Pamungkas Putri, Rohmad Yudi Utomo, Adam Hermawan, Edy Meiyanto. Combination of Cisplatin and Cinnamon Essential Oil Inhibits HeLa Cells Proliferation through Cell Cycle Arrest. J App Pharm Sci, 2014; 4 (12): 014-019.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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