Research Article | Volume: 4; Issue: 11, November, 2014

Improvement of cell mass production of Lactobacillus delbrueckii sp. bulgaricus WICC-B-02: A newly isolated probiotic strain from mother’s milk

Elsayed A. Elsayed ,  ,  Nor Zalina Othman,  Roslinda Malek , Tebbie Tang,  and HeshamA. El Enshasy ,    
Elsayed A. Elsayed1 , 2, Nor Zalina Othman3, Roslinda Malek3 ,Tebbie Tang3, and HeshamA. El Enshasy3 , 4

1Bioproducts Research Chair, Zoology Department, Faculty of Science, King Saud University, Kingdom of Saudi Arabia.

2Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.

3Institute of Bioproduct Development (IBD), UniversitiTeknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.

4City of Scientific Research and Technology Application, New Burg Al Arab, Alexandria, Egypt.

Open Access   

Published:  Nov 27, 2014

DOI: 10.7324/JAPS.2014.4112
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Abstract

Lactobacillus delbrueckii sp. bulgaricus WICC-B-02 is new probiotic strain which was initially isolated from the mother’s milk. As lactic acid bacterium, it’s known as a highly efficient probiotic microorganism with a wide range of benefits on the human health. This study was conducted to design and establish industrial platform for high cell mass production of L. delbrueckii sp. bulgaricus for pharmaceutical and food industries application. However, due to low cell mass production caused by the accumulation of lactic acid during the cultivation of lactic acid bacteria, therefore, the optimization of cell mass production with low lactic acid production was developed in this study. The new formulation of the production medium was developed by optimizing the main components such as glucose (30 g.L-1), yeast extract (6.0 g.L-1) and peptone (6.0 g.L-1)in shake flask cultivation. The cell mass production was 3.14 g.L-1, and it increased to 6.08g.L-1 with the lactic acid production being reduced by about 33%, compared to the un-optimized medium.


Keyword:     Probiotics Process development Mother’s milk Lactobacillus delbrueckii.

Citation:

Elsayed A. Elsayed, Nor Zalina Othman, Roslinda Malek, Tebbie Tang, HeshamA. El Enshasy. Improvement of cell mass production of Lactobacillus delbrueckii sp. bulgaricus WICC-B-02: A newly isolated probiotic strain from mother’s milk. J App Pharm Sci, 2014; 4 (11): 008-014.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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